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  5. Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0, for molecular

Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0, for molecular

Catalog No. AC327111000
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Quantity:
100 mL
500 mL
2.5 L

Description

Phenol-Chloroform-Isoamyl alcohol mixture in the ratio of 25:24:1 (v/v/v), CAS # 136112-00-0, 67-66-3, 123-51-3, is a solution used in the purification of nucleic acids. | CAS: 136112-00-0 | C6H6O

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

  • This solution contains a mixture of pure chloroform, isoamyl alcohol and phenol saturated with Tris-EDTA, with a stabilized pH of 8.0

Applications

  • Phenol-Chloroform-Isoamyl alcohol mixture solution (25:24:1) is used in the extraction of nucleic acids, DNA, and RNA
  • When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
  • It is also used to generate DNA minisatellite fingerprints
TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0
Color Yellow to Orange
Name Note DNase, RNase and Protease Free
CAS 136112-00-0, 67-66-3, 123-51-3
Molecular Formula C6H6O
MDL Number MFCD00133763
UN Number 2927
Merck Index 14, 7241
Density 1.2800g/mL
Formula Weight 94.11
Grade Molecular Biology
Packaging Glass bottle
Specific Gravity 1.28
Treatment(s) Stabilized
pH 7.8 to 8.2 (25°C)
Quantity 100 mL
Physical Form Solution
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RUO – Research Use Only

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