U-Blot Phospho-(Ser) 14-3-3 Binding Motif Rabbit mAb is a Rabbit-derived recombinant monoclonal antibody (clone W-R30) that specifically recognizes Phospho-(Ser) 14-3-3 Binding Motif a phosphorylation modification at Serine residues within the 14-3-3 binding motif It shows reactivity with Human Rat Validated applications include WB Recommended dilutions WB 11000 Purified by protein a The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival apoptosis proliferation and checkpoint control Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins Motif 1 (Arg/Lys and Ser at positions -3 and -2 phospho-Ser at position 0 and Pro at position 2) is found in critical regulatory proteins including Bad cdc25C FKHRL1 PKC and c-Raf Supplied as 100 uL