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Molecular Probes™ pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE)
Description
New pH-sensitive pHrodo™ Red dye conjugates give faster and more accurate results than any other phagocytosis assay
pHrodo™ Red dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes
Get faster staining and more accurate results - without the need for wash steps or quencher dye
• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments
• Multiplex with green dyes such as GFP, Fluo-4, or calcein
The fluorescence of the novel pHrodo™ Red dye dramatically increases as pH decreases from neutral to the acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.
Use the ready-made pHrodo™ Red E.coli BioParticles™ conjugates in imaging or flow applications, or pHrodo™ Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
Consult user Manual for solubility instructions.
Specifications
Specifications
| Quantity | 1 mg |
| Product Type | Succinimidyl Ester |
| Molecular Weight (g/mol) | ∼650 |
| Content And Storage | Store in freezer (-5°C to -30°C) and protect from light. |
| Chemical Reactivity | Amine |
| Shipping Condition | Room Temperature |
| Reactive Moiety | Active Ester, Succinimidyl Ester |
| Label or Dye | pHrodo™ Red |
| Label Type | Classic Dyes |
| Color | Red |
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For Research Use Only. Not for use in diagnostic procedures.
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