Thermo Scientific™ EZ-Link™ Activated Peroxidase Antibody Labeling Kit

Glutaraldehyde-activated horseradish peroxidase (HRP) enzyme and kit to label primary antibodies and purify the resulting antibody-HRP conjugates.

Supplier:  Thermo Scientific™ 31497

Catalog No. PI31497

Description

Thermo Scientific™ Pierce Activated HRP and Antibody Labeling Kit contains horseradish peroxidase enzyme that has been glutaraldehyde-activated to enable labeling and preparation of antibody conjugates.

This chemistry can be used to couple HRP to primary amines on another protein such as an antibody. The Thermo Scientific Activated Peroxidase Kit is designed to purify HRP-antibody conjugates by using Immobilized Protein A/G to separate unreacted enzyme from the antibody conjugate. Using Activated HRP produces an HRP-antibody conjugate in which both the enzyme activity and the antigen-binding activity are preserved.

Highlights:

• Reacts with primary amines to form a covalent amide bond
• No reduction step is necessary to secure the linkage
• Can be stored for at least 12 months at -20'C
• One mg produces about 0.5mL of conjugate with a working dilution of 1:1,000 when coupled to a high titer antibody
• Enzyme activity is > 200 units/mg

Includes:

5mg Activated Peroxidase, 50mL Conjugation Buffer, 250mg Lysine, 0.5mL Immobilized Protein A/G Column, 200mL Gentle Ag/Ab Binding Buffer, 200mL Gentle Ag/Ab Elution Buffer

Specifications

• Product Type: Antibody Labeling Kit
• Content And Storage: Sufficient For: 5 conjugations, each with 0.3 mg antibody (IgG)
• Activated Peroxidase, 5 mg
• Conjugation Buffer, 50 mL
• Lysine, 250 mg
• Protein A/G Column, 0.5 mL
• Gentle Ag/Ab Binding Buffer, 200 mL
• Gentle Ag/Ab Elution Buffer, 200 mL
  
  Store activated peroxidase at -20°C. Store other components at 4°C.
• Product Line: EZ-Link™
• Labeling Target: IgG

• Sufficient For: 5 conjugations, each with 0.3 mg antibody (IgG)
• Labeling Scale: 300 μg
• Label or Dye: HRP (Horseradish Peroxidase)

For Research Use Only. Not for use in diagnostic procedures.