Thermo Scientific™ Pierce™ Agarose ChIP Kit

Description

Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.

Thermo Scientific™ Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

This chromatin IP kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. The kit includes a complete Chromatin Prep Module (also available separately) to efficiently extract and solubilize crosslinked DNA-protein interaction complexes from cells, plus an immunoprecipitation module that contains high-capacity, ChIP-grade Protein A/G agarose.

Highlights:

• Simple and streamlined ChIP assay protocol (< 8 hours)
• Highly efficient isolation and lysis of nuclei
• Easy and reproducible enzymatic digestion
• Low-background and high-capacity Protein A/G agarose resin
• Highly specific positive control reagents included: RNA polymerase II antibody and GAPDH PCR primers
• Fast and reproducible spin-column format; columns and collection tubes included
• High-recovery DNA purification
• Choose a complete ChIP assay kit or the separate chromatin preparation module

Includes:

Kit contains the Chromatin Prep Module (Part No. 26158; 11 components) for chromatin extraction and a complete set of immunoprecipitation reagents (15 components) for IP experiments with controls

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Specifications

• Binding Capacity: >50 mg Human IgG/mL resin binding capacity
• Capacity (Metric): ≥50 mg Human IgG per mL Resin Binding
• Description: Pierce Agarose ChIP Kit
• Content And Storage: Sufficient For: 30 complete chromatin IP assay experiments• ChIP Grade Protein A/G Plus Agarose, 0.65 mL (store at 4°C)
  • IP Dilution/Wash Buffer (5X), 11 mL (store at 4°C)
  • IP Wash Buffer 3 (5X), 4.5 mL (store at 4°C)
  • IP Elution Buffer (2X), 4.5 mL (store at 4°C)
  • Column Accessory Pack, 3 packs (store at room temperature or 4°C)
  • Microcentrifuge Tubes (1.5mL), 75 tubes (store at room temperature or 4°C)
  • DNA Clean-Up Column, 40 columns (store at room temperature or 4°C)
  • DNA Column Binding Solution, 30 mL (store at room temperature or 4°C)
  • DNA Column Wash Solution, 6 mL (store at room temperature or 4°C)
  • pH Indicator, 0.8 mL (store at room temperature or 4°C)
  • DNA Column Elution Solution, 5 mL (store at room temperature or 4°C)
  • Anti-RNA Polymerase II Antibody, 25 μL (store at -20°C)
  • Normal Rabbit IgG (1 mg/mL), 10 μL (store at -20°C)
  • ChIP Positive Control Primers (GAPDH promoter, Human-specific), 100 μL (store at -20°C)
  • Pierce Chromatin Prep Module (Part No. 26158) (store Proteinase K and Micrococcal Nuclease at -20°C. Store all other components at 4°C).
• Product Line: Pierce™
• Quantity: 30 Reaction Kit
• Type: ChIP Kit
• Sufficient For: 30 Reactions
• Format: Kit

For Research Use Only. Not for use in diagnostic procedures.