Thermo Scientific™ Pierce™ Chromatin Prep Module

Description

Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.

Thermo Scientific™ Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

This chromatin IP kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. The kit includes a complete Chromatin Prep Module (also available separately) to efficiently extract and solubilize crosslinked DNA-protein interaction complexes from cells, plus an immunoprecipitation module that contains high-capacity, ChIP-grade Protein A/G agarose.

Highlights:

• Simple and streamlined ChIP assay protocol (< 8 hours)
• Highly efficient isolation and lysis of nuclei
• Easy and reproducible enzymatic digestion
• Low-background and high-capacity Protein A/G agarose resin
• Highly specific positive control reagents included: RNA polymerase II antibody and GAPDH PCR primers
• Fast and reproducible spin-column format; columns and collection tubes included
• High-recovery DNA purification
• Choose a complete ChIP assay kit or the separate chromatin preparation module

Includes:

Kit contains the Chromatin Prep Module (Part No. 26158; 11 components) for chromatin extraction and a complete set of immunoprecipitation reagents (15 components) for IP experiments with controls

Specifications

• Capacity (Metric): 2 x 10⁶ cells/reaction
• Description: Pierce Chromatin Prep Module
• Content And Storage: Sufficient For: 30 chromatin preparations for ChIP assays
  • Membrane Extraction Buffer, 10 mL
  • Nuclear Extraction Buffer, 10 mL
  • MNase Digestion Buffer, 5 mL
  • MNase Stop Solution, 0.5 mL
  • Halt Protease and Phosphatase Inhibitor Cocktail EDTA-free (100X), 4 x 90 μL
  • Sodium chloride (5M), 3 mL
  • Glycine solution (10X), 15 mL
  • PBS (20X), 15 mL
  • Micrococcal Nuclease (ChIP Grade) (10 U/μL) (store at -20°C)
  • Proteinase K (20 mg/mL), 0.25 mL (store at -20°C)
  • DTT, Lyophilized, 1 vial
  
  Unless otherwise indicated, store all components at 4°C.
• Product Line: Pierce™
• Quantity: 30 reactions
• Type: Chromatin Isolation Kit
• Sufficient For: 30 Reactions
• Format: Kit

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.