Thermo Scientific™ Proteinase K Solution, ChIP grade

Description

Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.

Thermo Scientific™ Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

This chromatin IP kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. The kit includes a complete Chromatin Prep Module (also available separately) to efficiently extract and solubilize crosslinked DNA-protein interaction complexes from cells, plus an immunoprecipitation module that contains high-capacity, ChIP-grade Protein A/G agarose.

Highlights:

• Simple and streamlined ChIP assay protocol (< 8 hours)
• Highly efficient isolation and lysis of nuclei
• Easy and reproducible enzymatic digestion
• Low-background and high-capacity Protein A/G agarose resin
• Highly specific positive control reagents included: RNA polymerase II antibody and GAPDH PCR primers
• Fast and reproducible spin-column format; columns and collection tubes included
• High-recovery DNA purification
• Choose a complete ChIP assay kit or the separate chromatin preparation module

Includes:

Kit contains the Chromatin Prep Module (Part No. 26158; 11 components) for chromatin extraction and a complete set of immunoprecipitation reagents (15 components) for IP experiments with controls

Specifications

• Description: Pierce Proteinase K
• Content And Storage: Upon receipt store at -20°C.
• Form: Liquid
• Quantity: 0.25 mL
• Type: Proteinase K (ChIP grade)

For Research Use Only. Not for use in diagnostic procedures.