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Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin
Description
Thermo Scientific Pierce Anti-DYKDDDDK Spin Columns provide a fast, convenient method for purification of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as 'FLAG', is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to UltraLink Biosupport, which is a rigid, hydrophilic, highly crosslinked, copolymeric, and porous resin with high coupling capacity.
For protein purification, resin is added to a sample containing DYKDDDDK-tagged proteins with the tag on either the N- or C-terminus. Following capture of DYKDDDDK-tagged proteins, non-specifically bound proteins can be washed away before dissociating bound target proteins with elution buffer.
Features of Pierce Anti-DYKDDDDK Affinity Resin
- Specific—unique base beads and highly specific antibody minimize off-target binding (low non-specific binding)
- High purity—optimized bind-wash-elute protocol enables high purity
- High yield—special antibody conjugation method enables high yield
- Rapid—entire purification protocol typically takes less than 40 mins
- Economical—purification protocol allows multiple reuses
- Flexible—available in multiple formats including bulk loose resin and spin columns
Characteristics of Pierce Anti-DYKDDDDK Affinity Resin
- Composition: anti-DYKDDDDK antibody covalently attached to UltraLink Biosupport, a highly crosslinked rigid support
- Particle size: 50–80 μm
- pH stability: 1–13
- Operating temperature: 2–30°C; do not freeze
- Particle concentration: 50% slurry in phosphate buffered saline, 0.02% sodium azide, pH 7.5
- Binding capacity: ≥3.0 mg DYKDDDDK-tGFP-His protein (∼32 kDa/mL settled resin)
Available formats
- Resin slurries—2 mL, 10 mL, and 100 mL of 50% slurry in PBS with 0.02% sodium azide, pH 7.5
- Spin columns—10 mL spin columns containing 3 mL of settled resin, supplied at 6 mL 50% slurry
Specifications
Specifications
| Product Line | Pierce™ |
| Product Type | Affinity Resin |
| Quantity | 2 mL |
| Formulation | 50% suspension in PBS containing 0.02% Sodium azide |
| Content And Storage | 1 mL settled resin. 2 mL supplied as a 50% slurry in PBS containing 0.02% sodium azide. Store at 2–8°C. |
| Format | Liquid |
| Stationary Phase | Anti-DYKDDDDK monoclonal antibodies |
| Shipping Condition | Wet Ice |
| Form | Liquid |
| Matrix | Affinity Resin |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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