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Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Catalog No. PIA36797
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Quantity:
4 mL
20 mL

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

Thermo Scientific Pierce Anti-DYKDDDDK Magnetic Agarose provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins from in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as 'FLAG', is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to a magnetite-embedded agarose core particle.

For protein purification, the magnetic agarose is added to a sample containing DYKDDDDK-tagged proteins with the tag on either the N- or the C-terminus. Captured proteins are then magnetically separated from the supernatant, and non-specifically bound proteins can be washed away before dissociating bound DYKDDDDK-tagged proteins with elution buffer. The magnetic agarose is removed from the solution using a magnetic stand or an instrument such as the KingFisher Flex Magnetic Particle Processor. Automated instruments are especially useful for higher throughput purifications and screening of purification conditions.

Features include:

  • Specific—unique base beads and highly specific antibody minimizes off-target binding (low non-specific binding)
  • High purity—optimized bind-wash-elute protocol enables high purity
  • High yield—special antibody conjugation method enables high yield
  • Rapid—entire purification protocol typically takes less than 40 mins
  • Economical—purification protocol allows multiple reuses
  • Versatile—beads are compatible with manual and automated workflows (e.g., KingFisher instruments)

Characteristics of Pierce Anti-DYKDDDDK Magnetic Agarose:

  • Composition: anti-DYKDDDDK antibody covalently attached to a magnetic, highly crosslinked agarose support
  • Magnetization: ferrimagnetic with low remanence
  • Bead size: 10–40 μm
  • Bead concentration: 25% slurry in phosphate buffered saline, 0.01% Tween-20 detergent, 0.02% sodium azide, pH 7.2
  • Binding capacity: ≥3.2 mg DYKDDDDK-tGFP-His protein (∼32 kDa)/mL settled beads
TRUSTED_SUSTAINABILITY

Specifications

Product Line Pierce™
Product Type Magnetic Agarose
Bed Volume 1 mL
Quantity 4 mL
Particle Size 25 to 30 μm
Formulation Slurry: 25%
Content And Storage 1 mL settled beads. 4 mL supplied as a 25% suspension in PBS containing 0.02% sodium azide.

Store at 2–8°C.
Capacity 4 mL
Format Liquid
Stationary Phase Anti-DYKDDDDK monoclonal antibodies
Shipping Condition Wet Ice
Form Liquid
Matrix Magnetic Agarose
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WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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