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Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads
Description
Thermo Scientific Pierce Anti-HA Magnetic Beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.
Features of Anti-HA Magnetic Beads:
- Specific—highly specific anti-HA monoclonal antibody (clone 2-2.2.14) enables high yield and high purity immunoprecipitation
- Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour
- Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for HA-tag IP or co-IP experiments
- Versatile—beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)
The blocked magnetic bead surface is coated with anti-HA antibody, a highly specific mouse IgG1 monoclonal antibody that recognizes the HA-epitope tag (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein. The Pierce Anti-HA Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.
Specifications:
Product Details:
Pierce Anti-HA Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant HA-tagged proteins and the co-immunoprecipitation (co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing HA-tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine, pH 2.0, 50 mM NaOH, or SDS-PAGE sample buffer. If gentler elution conditions are desired, 2 mg/mL Pierce HA peptide can be used. The protocol has been optimized for each of these conditions. Anti-HA antibody can be used to detect HA-tagged protein by Western blot analysis.
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Specifications
Specifications
| Capacity (Metric) | 1 mL |
| Concentration | Slurry: 10mg/mL, 1% solids |
| Content And Storage | Store at 4°C. |
| Particle Size | 1 μm |
| Form | Liquid |
| Product Line | Pierce |
| Quantity | 1 mL |
| Protein Form | Recombinant |
| Target | HA |
| Type | Magnetic Affinity Bead |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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