Thermo Scientific™ Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible

Catalog No. PI23250

Supplier: Thermo Scientific™ 23250

Description

Reducing agent-compatible version of our popular BCA Reagent Kit to measure protein concentration in samples containing DTT or BME thiol-reductants.

This reducing agent-compatible (RAC) version of the Thermo Scientific™ Pierce BCA Protein Assay provides accurate determination of protein concentration in samples that contain dithiothreitol (DTT), 2-mercaptoethanol (BME), TCEP and other disulfide reducing agents.

The BCA Protein Assay Kit - Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Highlights:

• Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP

• BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required

• Small samples – requires only 25μL (standard kit) or less than 10μL (microplate kit) of sample

• Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)

• Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods

• High linearity – linear working range for BSA equals 125 to 2000μg/mL

Includes:

BCA Reagents A and B, Compatibility Reagent, Reconstitution Buffer, and Albumin Standard (2mg/mL); Microplate kit also includes assay-compatible 96-well microplates

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Specifications

• Content And Storage: Sufficient For: 250 tube assays with 25 μL samples
  • BCA Reagent A, 250 mL
  • BCA Reagent B, 25 mL
  • Compatibility Reagent, 10 x 20 mg
  • Reconstitution Buffer, 15 mL
  • Albumin Standard Ampules, 2mg/mL, 10 x 1 mL
  
  Store at room temperature
• Description: Pierce BCA Tube Protein Assay Kit, Reducing Agent Compatible (RAC)
• Assay: BCA Assay
• Detection Method: Colorimetric
• Product Line: Pierce™
• Product Type: Protein Quantitation Assay
• Specificity: Not Target-Specific
• Sufficient For: 250 Tube Assays
• For Use With (Application): Solution-based Detection, Absorbance
• For Use With (Equipment): Spectrophotometer, Microplate Reader
• Quantity: 275 mL

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.