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Thermo Scientific™ Pierce™ Boronic Acid Resin

Selectively bind and remove or purify ribonucleotides and small oligonucleotide RNAs using polyacrylamide beads of immobilized m-aminophenyl-boronic acid.

Supplier:  Thermo Scientific™ 20244

Catalog No. PI20244


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Description

Description

Perform ribonucleotide and oligonucleotide RNA isolation with Thermo Scientific™ Pierce Boronic Acid Resin.

This immobilized boronic acid is an easy-to-use polyacrylamide affinity support for purification of ribonucleotides and other small molecular weight compounds that contain cis-diol groups. The ligand (m-aminophenyl-boronic acid) binds to the cis-diol groups on the sugar portion of nucleotides, forming a reversible five-member ring complex. After washing away non-bound molecules from the sample, the complex can be dissociated and the nucleotide eluted by lowering the pH or by addition of sorbitol.

Highlights:

  • Ligand – boronic acid

  • Support – beaded polyacrylamide (1800 MW exclusion limit)

  • Spacer – m-aminophenyl group

  • Loading – 100μmol boronate per milliliter of settled resin

  • Capacity – at least 99% binding and recovery of 110μmol AMP per milliliter of settled resin

  • Format – 50% resin slurry in 0.05% sodium azide

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Store at 4°C.
Pierce™
Ribonucleotide
Boronic Acid
Affinity, Polyacrylamide Resin
Resin Slurry
10 mL
Resin
Bottle
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For Research Use Only. Not for use in diagnostic procedures.