Thermo Scientific™ Pierce™ C18 Spin Tips & Columns

Catalog No.	Quantity	Product Type
PI87784	96 x 100 μL Tips	Pipette Tip
PI89870	25 Columns	Column
PI89873	50 Columns	Column
PI84850	96 Tips	Spin Tip
PI87782	96 x 10 μL Tips	Pipette Tip

Catalog No. PI87784 Supplier Thermo Scientific™ Supplier No. 87784

Description

Thermo Scientific Pierce C18 Spin Columns (10–150 μL) and Spin Tips (10–100 μL) effectively remove interfering contaminants, while also maintaining excellent binding and recovery of peptide samples for use upstream of mass spectrometry and other high sensitivity detection methods.

C18 Spin Tips for Peptide Desalting and Cleanup

Pierce™ C18 spin tips are designed for rapid, reproducible peptide desalting, cleanup, and concentration prior to mass spectrometry analysis. Utilizing reversed-phase C18 chemistry, these tips help enable efficient peptide binding and recovery while removing salts, detergents, and other contaminants that can interfere with downstream applications.

Used in combination with Pierce Spin Tip Adaptors (Catalog No. A40011776) and low protein binding microcentrifuge tubes (Catalog No. 90410), C18 spin tips support a simple centrifugation-based workflow that delivers consistent and reliable results across a wide range of sample inputs.

Features of C18 Spin Tips

• Reliable – proven reversed-phase C18 chemistry
• Efficient – effectively removes interfering contaminants to improve spectral quality
• Streamlined – simple workflow using tip adaptor and centrifugation steps

Applications

• Peptide desalting prior to LC-MS analysis
• Cleanup following protein digestion workflows
• Removal of salts, buffers, and contaminants
• Peptide concentration and sample preparation

How to Use

C18 spin tips use a bind–wash–elute workflow performed by centrifugation. Tips are placed into a tip adaptor seated in a microcentrifuge tube for each step. Simply activate and equilibrate prior to adding your acidified sample. To enhance peptide binding repeat the sample loading step one time. Lastly, wash 2-3 times and elute into a fresh tube using an acetonitrile solution. Refer to Table 1 in the Figures for recommended buffers, volumes and spin speeds for each tip size.

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Specifications

• Bed Volume: 100 μL
• Description: Pierce C-18 Tips, 100 μL bed
• For Use With (Equipment): Microcentrifuge, Mass Spectrometer
• Product Type: Pipette Tip
• Product Line: Pierce
• Content And Storage: Store at room temperature.
• Starting Material: Peptides, Protease-digested Protein
• Final Product Type: Peptides
• Detection Method: Mass Spectrometry
• Format: Tip
• Workflow Step: Sample Clean Up
• Quantity: 96 x 100 μL Tips