Thermo Scientific™ Chemiluminescent Nucleic Acid Detection Module Kit

Description

Detect biotin-labeled nucleic acid probes in various membrane blotting applications with this complete chemiluminescent detection system.

Thermo Scientific™ Pierce Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acid probes in various membrane blotting applications.

The Thermo Scientific Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acids for various blotting applications including Northern/Southern blots, ribonuclease protection assays (RPAs) and electrophoretic mobility shift assays (EMSAs). This module provides the essential detection components used in the Thermo Scientific North2South and LightShift EMSA Kits. The system includes an enhanced luminol substrate for horseradish peroxidase (HRP) with optimized blocking and wash buffers that together produce sensitivity equivalent to radioactive (³²P) systems. Obtain publication-quality blots having excellent signal and low background.

Highlights:

• Fast and sensitive – achieve the same speed and detection levels you've come to expect with the North2South and LightShift Kits

• Versatile – kit protocol (included) is applicable to any biotin-based nucleic acid detection system: Northerns, Southerns, gel shifts, RPAs and colony lifts

• Flexible – kit contains only the critical detection components for those who already have biotin-labeled probes and an optimized hybridization method developed

Includes:

Stabilized Streptavidin-HRP Conjugate 1.5mL, Luminol/Enhancer Solution 80mL, Stable Peroxide Solution 80mL, Blocking Buffer 500mL, 4X Wash Buffer 500mL, Substrate Equilibration Buffer 500mL

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Specifications

• Content And Storage: Sufficient For: 800 cm² of membrane (10 mini-gel blots)
  • Stabilized Streptavidin-HRP, 1.5 mL
  • Luminol/Enhancer Solution, 80 mL
  • Stable Peroxide Solution, 80 mL
  • Blocking Buffer (Part No. 89880A), 500 mL
  • Wash Buffer (4X), 500 mL
  • Substrate Equilibration Buffer, 500 mL
  
  Store at 4°C.
• Detection Method: Chemiluminescence
• Format: Kit
• Product Line: Pierce™
• Product Type: Nucleic Acid Detection Module Kit
• Quantity: 10 Blots

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.