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Thermo Scientific™ Pierce™ Chicken IgY Purification Kit

Purify chicken antibodies from hen egg yolks with a unique delipidation reagent and precipitation reagent to selectively isolate the IgY fraction.

Supplier:  Thermo Scientific™ 44918

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Catalog No. PI44918


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Description

Description

Thermo Scientific™ Pierce Chicken IgY Purification Kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.

The IgY Purification Kit is simple and easy to use. After isolating a yolk using the supplied egg separator, lipids are effectively removed by incubation and centrifugation in Pierce Delipidation Reagent. This first reagent is also effective as a storage solution, enabling multiple yolks to be collected and stored for simultaneous extraction at a later date. Upon addition of the specialized Pierce IgY Precipitation Reagent and subsequent centrifugation, a nearly pure pellet of intact IgY results; it is easily dissolved in phosphate buffered saline (PBS) or other storage buffer. The purified IgY is ready for use in immunodetection methods or antigen-specific purification without interference from lipids.

Highlights:

  • Cost-effective – purifies twice as much IgY per egg than other popular kits, providing a lower cost-per-milligram of purified IgY

  • High purity – method yields chicken antibody that is 85 to 95% pure (based on SDS-PAGE analysis)

  • Easy to use – simple precipitation method purifies chicken IgY without affinity columns

  • Flexible protocol – eggs can be harvested and quickly stored in buffer for purification at a later date

  • Convenient – use eggs directly out of refrigerator; no need to equilibrate them to room temperature

Includes:

Egg separator, delipidation reagent and IgY precipitation reagent

TRUSTED_SUSTAINABILITY
Specifications

Specifications

IgY Purification Kit
Precipitation
IgY Antibody
Sufficient For: Purifying chicken IgY from 5 egg yolks
• Delipidation Reagent, 500 mL
• IgY Precipitation Reagent, 500 mL
• Egg Separator, 1 separator

Store at 4°C.
Pierce™
Kit, Bottle
Pierce Chicken IgY Purification Kit
1 L
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For Research Use Only. Not for use in diagnostic procedures.