Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ Classic Magnetic IP/Co-IP Kit
GSA_VA

Catalog No. PI88804
Click to view available options
Quantity:
40 reactions kit

Easily immunoprecipitate target protein complexes by magnetic separation using high-quality Protein A/G magnetic beads and optimized buffers.

The Thermo Scientific Pierce Classic Magnetic IP/Co-IP Kit enables highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of antigens and protein-complexes using less than 10 μg of antibody and a magnetic separator.

Features of the Classic Magnetic IP/Co-IP Kit:

  • Compatible—magnetic beads based on Protein A/G recombinant protein ensures compatibility with most primary antibodies, whether from mouse or rabbit
  • Fast—immunoprecipitate in as few as 30 minutes to reduce background and improve the capture of transient protein complexes
  • Clean—immobilize your antibody to prevent contamination in your eluate
  • Resistant—no leaching of Protein A/G in the presence of detergents, low pH buffers or common mass spectrometry solvents
  • Efficient—immunoprecipitate with half the recommended volume of magnetic particles compared to other magnetic beads
  • Convenient—the comprehensive Co-IP kit contains the magnetic beads and all essential buffers to complete immunoprecipitation experiments

This immunoprecipitation kit uses high-quality Thermo Scientific Pierce Protein A/G Magnetic Beads and optimized buffers and flexible protocol to accomplish high-yield IP or co-IP with manual or automated magnetic-separation tools. The optimized Lysis/Wash Buffer optimizes yield and efficient binding of antibody-antigen and co-IP interactions. The relatively gentle, low-pH Elution Buffer dissociates the bound immune complex from the Protein A/G. Alternatively, the included Lane Marker Sample Buffer provides rapid, denaturing elution for direct SDS-PAGE analysis of IP products.

Includes:

Pierce Protein A/G Magnetic Beads, lysis/wash buffer, elution buffer, neutralization buffer and sample loading buffer

Immunoprecipitation with magnetic particles is performed much like IP with beaded agarose, except that separations are performed using a magnet rather than by centrifugation. The specific antibody is first added to the sample to form an immune complex that is then bound to the magnetic beads. The complex is washed to remove non-bound material and a low-pH elution buffer dissociates the bound immune complex from the Protein A/G. Alternatively, the Lane Marker Sample Buffer is included for dissociation using denaturing conditions or for downstream sample prep for SDS-PAGE analysis. The kit includes Thermo Scientific Pierce Protein A/G Magnetic Beads for fast and convenient magnetic isolation of antigens and optimized buffers for high antigen yield. The beads are removed from the solution manually using a magnetic stand or by automation with an instrument such as the Thermo Scientific KingFisher Flex Instrument.

It is generally known that longer antibody-sample incubations times (2 hours to overnight) usually provide greater yields in IP assays. It is often assumed that this greater yield comes with increased background. Our researchers have found that overnight antibody-sample binding followed by immunoprecipitation with the Pierce Classic Magnetic IP/Co-IP Kit does tend to increase yield but does not increase background. Thus, we recommend that researchers perform this binding step for at least 1 hour whenever possible.

Protocol Summary:

  • Incubate cell lysate with IP antibody for 1 to 2 hours at room temperature or overnight at 4°C.
  • Bind antigen-antibody complex to Protein A/G magnetic beads for 1 hour at room temperature.
  • Wash beads twice with IP Lysis/Wash Buffer and once with purified water.
  • Elute the antigen/antibody complex.

Related Products

Pierce™ Classic IP Kit (Catalog #26146)

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

TRUSTED_SUSTAINABILITY

Specifications

Capacity (Metric) 1 mL
Formulation Slurry: 10 mg/mL, 1% solids
Content And Storage Sufficient For: 40 IP reactions using 25 μL of beads
• Pierce Protein A/G Magnetic Beads, 1 mL
• Pierce IP Lysis/Wash Buffer, 2 x 50 mL
• Lane Marker Sample Buffer (5X), 5 mL
• Elution Buffer, 5 mL
• Neutralization Buffer, 0.5 mL

Store at 4°C.
Assay Affinity Purification
For Use With (Equipment) KingFisher™ Flex, Magnetic Stand
Product Line Pierce™
Quantity 40 reactions kit
For Use With (Application) Immunoprecipitation (IP)
Target Not Target-Specific
Type Immunoprecipitation/Co-Immunoprecipitation Kit
Sufficient For 40 Reactions
Format Kit
Show More Show Less
WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.