Learn More
Thermo Scientific™ Pierce™ Co-Immunoprecipitation Kit

Description
Thermo Scientific™ Pierce Co-Immunoprecipitation Kit provides all the components necessary to perform a properly controlled co-IP experiment and obtain purified products without antibody contamination.
Highlights:
Works for any purified antibody – perform co-immunoprecipitation experiments using any purified antibody regardless of species or Ig class (chicken IgY, human IgE, mouse IgG1, IgM, etc.); no dependence on Protein A and Protein G affinity binding
No antibody interference – covalent attachment method and non-reducing elution system yields pure co-IP products without contamination by the IP antibody
Reusable antibody beads – covalent antibody immobilization allows prepared IP resin to be reused and provides potential savings of costly antibody
Complete Kit – all reagents for antibody immobilization and many IP experiments, including binding and wash buffers, elution buffer and convenient microcentrifuge spin columns to make resin manipulations easier and more efficient
Control beads included – underivatized agarose beads provided for use as negative control for nonspecific binding
Versatile – co-IP method is compatible with any physiolgical (non-denaturing) protein sample buffer that is compatible with the specific antibody-antigen and protein interaction complex
Includes:
AminoLink Plus Coupling Resin, coupling buffer, quenching buffer, wash solution, sodium cyanoborohydride solution, IP lysis/wash buffer, conditioning buffer, Tris-buffered saline, elution buffer, lane marker sample buffer, control agarose resin, spin columns and collection tubes
This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications
Specifications
Content And Storage | Sufficient For: Preparing and performing 50 co-IP reactions, each with 25 μL of resin • AminoLink Plus Coupling Resin, 2 mL • Coupling Buffer (20X), 25 mL • Sodium Cyanoborohydride Solution (5M), 0.5 mL • Quenching Buffer, 50 mL • Wash Solution, 60 mL • IP Lysis/Wash Buffer, 2 x 50 mL • Modified Dulbecco's PBS (20X), 25 mL • Conditioning Buffer (100X), 5 mL • Elution Buffer, 50 mL • Lane Marker Sample Buffer (5X), 5 mL • Pierce Spin Columns, Screw Cap, 50 columns • Microcentrifuge Collection Tubes, 2 mL, 100 tubes • Microcentrifuge Collection Tubes, 1.5 mL, 50 tubes • Pierce Control Agarose Resin, 2 mL Store at 4°C. |
Assay | Affinity Purification |
For Use With (Equipment) | Microcentrifuge |
Product Line | Pierce™ |
Quantity | 50 reactions kit |
For Use With (Application) | Immunoprecipitation (IP) |
Target | Not Target-Specific |
Type | Immunoprecipitation/Co-Immunoprecipitation Kit |
Sufficient For | 50 Reactions |
Format | Kit |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.