Thermo Scientific™ Pierce™ Co-Immunoprecipitation Kit

Description

Covalently immobilize IP antibodies and perform co-IP assays in which target protein complexes are immunoprecipitated without antibody contamination.

Thermo Scientific™ Pierce Co-Immunoprecipitation Kit provides all the components necessary to perform a properly controlled co-IP experiment and obtain purified products without antibody contamination.

The Co-Immunoprecipitation (Co-IP) Kit enables isolation of native protein complexes from a lysate or other complex mixture by directly immobilizing purified antibodies onto an agarose support. Co-IP is a common approach to study protein:protein interactions that uses an antibody to immunoprecipitate the antigen (bait protein) and co-immunoprecipitate any interacting proteins (prey proteins). Traditional co-IP methods that use Protein A or G result in co-elution of the antibody heavy and light chains that may co-migrate with relevant bands, masking important results. The Pierce Co-IP Kit resolves this issue by covalently coupling antibodies onto an amine-reactive resin. The kit includes optimized buffers for protein binding and recovery, reagents to perform control experiments and efficient spin columns and collection tubes, which shorten the protocol and minimize handling and mixing.

Highlights:

• Works for any purified antibody – perform co-immunoprecipitation experiments using any purified antibody regardless of species or Ig class (chicken IgY, human IgE, mouse IgG1, IgM, etc.); no dependence on Protein A and Protein G affinity binding

• No antibody interference – covalent attachment method and non-reducing elution system yields pure co-IP products without contamination by the IP antibody

• Reusable antibody beads – covalent antibody immobilization allows prepared IP resin to be reused and provides potential savings of costly antibody

• Complete Kit – all reagents for antibody immobilization and many IP experiments, including binding and wash buffers, elution buffer and convenient microcentrifuge spin columns to make resin manipulations easier and more efficient

• Control beads included – underivatized agarose beads provided for use as negative control for nonspecific binding

• Versatile – co-IP method is compatible with any physiolgical (non-denaturing) protein sample buffer that is compatible with the specific antibody-antigen and protein interaction complex

Includes:

AminoLink Plus Coupling Resin, coupling buffer, quenching buffer, wash solution, sodium cyanoborohydride solution, IP lysis/wash buffer, conditioning buffer, Tris-buffered saline, elution buffer, lane marker sample buffer, control agarose resin, spin columns and collection tubes

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Specifications

• Content And Storage: Sufficient For: Preparing and performing 50 co-IP reactions, each with 25 μL of resin
  • AminoLink Plus Coupling Resin, 2 mL
  • Coupling Buffer (20X), 25 mL
  • Sodium Cyanoborohydride Solution (5M), 0.5 mL
  • Quenching Buffer, 50 mL
  • Wash Solution, 60 mL
  • IP Lysis/Wash Buffer, 2 x 50 mL
  • Modified Dulbecco's PBS (20X), 25 mL
  • Conditioning Buffer (100X), 5 mL
  • Elution Buffer, 50 mL
  • Lane Marker Sample Buffer (5X), 5 mL
  • Pierce Spin Columns, Screw Cap, 50 columns
  • Microcentrifuge Collection Tubes, 2 mL, 100 tubes
  • Microcentrifuge Collection Tubes, 1.5 mL, 50 tubes
  • Pierce Control Agarose Resin, 2 mL
  
  Store at 4°C.
• Assay: Affinity Purification
• For Use With (Equipment): Microcentrifuge
• Product Line: Pierce™
• Quantity: 50 reactions kit
• For Use With (Application): Immunoprecipitation (IP)
• Target: Not Target-Specific
• Type: Immunoprecipitation/Co-Immunoprecipitation Kit
• Sufficient For: 50 Reactions
• Format: Kit

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.