Thermo Scientific™ Compat-Able™ Bradford Plus Protein Assay Kit

Description

Precipitation reagents to eliminate interfering substances from protein assay samples to make them compatible with BCA and Coomassie-Bradford assays.

Thermo Scientific™ Compat-Able Protein Assay Preparation Set eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.

The Compat-Able Reagents remove salts, detergents, reducing agents and other substances from protein samples to eliminate interference with protein assays. The protocol selectively precipitates all of the protein, thereby allowing the non-protein components to be easily decanted and removed. The purified, precipitated protein is redissolved in water, making it ready for accurate determination of the protein concentration by standard protein assay methods. The procedure is accomplished in less than 10 minutes with just a few simple steps. The Compat-Able Preparation Set is available individually or conveniently bundled with either the Thermo Scientific Pierce BCA Protein Assay Kit or the Thermo Scientific Pierce Coomassie Plus Protein Assay Kit.

Highlights:

• Eliminate incompatibilities – removes nearly any substance that interferes with colorimetric protein assays: salts, detergents, denaturants, reducing agents, and even IEF sample buffer

• Simple and fast – prepare samples in less than 10 minutes with four simple steps

• Convenient – reagent set is easier to use than homemade TCA or acetone precipitation reagents, thereby producing more consistent results

• Scalable – microcentrifuge method is easily scaled to larger centrifuge tube volumes for special needs

• Validated – tested and offered together with BCA and Coomassie Plus Protein Assay Kits; protocol integrates control and normalization procedures

Includes:

Set contains two bottled reagents (Reagent 1 and Reagent 2); Complete kits contain the Reagent Set plus components of the respective protein assay

Specifications

• Content And Storage: Sufficient For: Treating up to 500 protein assay samples and performing 630 tube assays or 3160 microplate assays
  • Compat-Able Reagent 1, 250 mL
  • Compat-Able Reagent 2, 250 mL
  • Pierce Coomassie Plus Assay Reagent, 950 mL
  • Albumin Standard Ampules, 2 mg/mL, 10 x 1 mL
• Description: Compat-Able Coomassie Plus Protein Assay Kit
• Assay: Bradford Assay
• Detection Method: Colorimetric
• Product Line: Compat-Able™
• Product Type: Protein Quantitation Assay
• Specificity: Not Target-Specific
• Sufficient For: 630 Tube Assays or 3160 Microplate Assays
• For Use With (Application): Absorbance, Solution-based Detection, Incompatibility Elimination
• For Use With (Equipment): Spectrophotometer, Microplate Reader
• Quantity: 1.5 L

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.