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Thermo Scientific™ Pierce™ Conjugate Purification Kit

Gently purify enzyme-conjugated antibodies from unreacted horseradish peroxidase (HRP) or alkaline phosphatase (AP) after labeling.

Manufacturer:  Thermo Scientific™ 44920

Catalog No. PI44920

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Thermo Scientific™ Pierce Conjugate Purification Kit is a unique affinity chromatography system for purifying horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated antibodies from unreacted enzyme after labeling.

The metal chelate ligand on the column binds the enzyme conjugates while allowing free enzyme to pass through.


  • Reduced background for blotting and ELISA
  • Fewer washes needed to remove background
  • Increased sensitivity of assay system with more active reagent
  • Improved signal:noise ratio of conjugate


Immobilized Iminodiacetic acid 1 × 2mL column, Polyacrylamide Desalting Column 1 × 10mL column, FreeZyme Column Activator Buffer 25mL, FreeZyme Elution Buffer 35mL, BupH™ Tris Buffered Saline Pack two each, BupH Phosphate Buffered Saline Pack one each, Activity Preservation Solution 1mL



Antibodies (IgG)
0.5-3 mg
10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibody
HRP (Horseradish Peroxidase)
Safety and Handling

Safety and Handling

Product Certifications


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