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Thermo Scientific™ Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit
Description
The Thermo Scientific™ Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.
The Pierce Cypridina-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Cypridina and Red Firefly luciferase activity. Cypridina luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. This reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching.
The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.
Highlights
- Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
- Sensitive—measure bright blue Cypridina and red firefly luciferase activities in the same sample
- Fast—no quenching step required, unlike in traditional sequential dual assays
- Multiplex—capable of quantitating two cellular activities in the same sample(s)
Requires
Cypridina and Red Firefly luciferase reporters; filter sets in the ranges of 425 to 525 nm for Cypridina Luc and 615 to 675nm for Red Firefly Luc; luminometer or other instrument capable of monitoring luminescence, such as the Varioskan ALF or the Varioskan LUX microplate readers; injector for assessing more than 24 wells at a time
Recommended applications
Study two regulatory elements at the same time; monitor two signaling pathways simultaneously; enable studying more than one target per screen (e.g., off-target effects)
Specifications
Specifications
| Type | Assay Kit |
| Target | Luciferase, Cypridina Luciferase |
| Sufficient For | 100 Dual Luciferase Assays |
| Quantity | 100 Reaction Kit |
| Content And Storage | Sufficient For: 100 dual-luciferase assays in microplate wells • Cypridina-Firefly Dual Assay Buffer, 5 mL (store at 4°C) • 100X Vargulin, 50 μL (store at -20°C) • D-Luciferin, 3 mg (store at 4°C) • Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature) Upon receipt, store kit at -20°C, or store individual components as indicated above. |
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Compatible Cells | Mammalian Cells |
| Detection Method | Bioluminescence |
| For Use With (Equipment) | Luminometer (Microplate) |
| Format | 384-well plate, 96-well plate |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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