Thermo Scientific™ iodoTMTsixplex™ Isobaric Label Reagents and Kits

Use Iodoacetyl Tandem Mass Tag (iodoTMT) Isobaric Label Reagents to label sulfhydryl-containing peptides for the multiplexed detection and quantitation of cysteine modification using tandem mass spectrometry analysis.

Catalog Number	Mfr. No.	Description
PI90100	Thermo Scientific™ 90100	iodoTMTzero™ Label Reagent, 5 x 0.2 mg
PI90101	Thermo Scientific™ 90101	iodoTMTsixplex™ Isobaric Label Reagent Set, 1 x 0.2 mg
PI90102	Thermo Scientific™ 90102	iodoTMTsixplex™ Isobaric Label Reagent Set, 5 x 0.2 mg

Description

Thermo Scientific iodoTMT Label reagents enable multiplexed identification and quantitation of cysteine containing peptides in different samples using tandem mass spectrometry. IodoTMT reagents are sets of isobaric (mass and structure) isomers that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (–SH) groups. iodoTMTzero Label Reagent allows testing and optimization of sample preparation, labeling, fractionation, and MS fragmentation for peptide identification and reporter detection without using the more costly isotope-labeled compounds.

Iodoacetyl tandem mass tag reagents enable selective labeling of cysteine-containing peptides for multiplexed sample quantitation using LC-MS/MS analysis. Similar to iodoacetamide, iodoTMT reagents react specifically with reduced cysteines (Cys) in peptides and proteins and enable quantitation of the relative abundance of cysteine modifications, such as S-nitrosylation and the oxidation of disulfide bonds. IodoTMT reagents irreversibly label free sulfhydryl groups on cysteine residues, in contrast to previous reversible cysteine-reactive TMT (cysTMT) reagents.

To selectively analyze the cysteine-labeled peptides, the sample may be enriched using Immobilized Anti-TMT Antibody Resin (Cat. No. 90076) and TMT Elution Buffer (Cat. No. 90104). This approach of selective thiol-labeling and affinity enrichment is similar to isotope-coded affinity tags (ICAT method) but allows for affinity enrichment with a non-biological tag and higher multiplex quantitation.

Features of iodoTMT reagents
• Specific—only reacts with sulfhydryl groups (reduced, unmodified cysteine residues)
• Irreversible—labeled proteins and peptides are not susceptible to reducing agents
• Flexible—options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation
• Versatile—can be used to study cysteine modifications (e.g., di-sulfide bridges, S-nitrosylation)

Specifications

• For Use With (Equipment): Mass Spectrometer
• Starting Material: Cell Lysate
• Detection Method: Mass Spectrometry

• Label or Dye: Tandem Mass Tag™ (TMT) Labels
• Final Product Type: Protein
• Workflow Step: Protein Labeling