Thermo Scientific™ Pierce™ Direct Magnetic IP/Co-IP Kit

Conjugate any antibody or protein to magnetic beads and immunoprecipitate (affinity purify) target protein complexes without IP-antibody interference.

Manufacturer:  Thermo Scientific™ 88828

Catalog No. PI88828

Description

The Thermo Scientific™ Pierce Direct Magnetic IP/Co-IP Kit uses NHS-chemistry to covalently immobilize IP antibodies onto magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

Magnetic IP with NHS chemistry is convenient and allows for the immobilization of IP antibody from any species and subtype without the variations in coupling efficiency seen with Protein A and Protein G. The IP antibody is covalently attached so target proteins or co-IP protein complexes can be eluted and analyzed without antibody contamination. The kit contains an optimized protocol and all buffers and reagents necessary for antibody coupling and high yield IP or co-IP using either manual magnetic stands or automated platforms such as the Thermo Scientific KingFisher Instruments.

Highlights:

• Antibody-free IP antibody is irreversibly attached to the magnetic beads, resulting in negligible co-elution of intact antibody or heavy and light chains with the purified antigen

• Fast complete antibody conjugation and immunoprecipitation in less than 4 hours

• Convenient the IP/co-IP kit contains magnetic beads and all essential buffers for optimal immunoprecipitation

• Antibody compatible use with any antibody species, class or isotype

• Low nonspecific binding the bead surface is pre-blocked and all nonreacted NHS-ester groups are fully quenched

• Protocol-compatible protein coupling to the beads and immunoprecipitation can be performed manually or by automation (e.g., Thermo Scientific KingFisher Instruments)

• Scalable use only the amount of antibody needed for a single IP experiment or prepare a larger quantity of antibody magnetic beads for multiple experiments

Includes:

NHS-Activated Magnetic Beads, IP lysis/wash buffer, elution buffer, neutralization buffer, quenching buffer, borate buffer and sample loading buffer

Specifications

• Assay: Affinity Purification
• For Use With (Equipment): KingFisher™ Flex, Magnetic Stand
• Target Specificity: Not Target-Specific

• Content And Storage: Sufficient For: 40 IP reactions using 25 µL of beads
  • Pierce NHS-Activated Magnetic Beads, 1 mL
  • IP Lysis/Wash Buffer, 2 x 50 mL
  • Elution buffer, pH 2.0, 5 mL
  • Lane Marker Sample Buffer, Non-reducing, (5X), 5 mL
  • Neutralization Buffer, pH 8.5, 0.5 mL
  • 0.67M Borate Buffer, 1 mL
  • BupH Borate Buffer Pack, 1 pack
  • Quenching Buffer, 25 mL
  
  Store at 4°C.
• Product Line: Pierce™
• Technique: Pull-Down, Immunoprecipitation (IP)