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Thermo Scientific™ Pierce™ Biotin 3' End DNA Labeling Kit

Description
Thermo Scientific™ Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.
Highlights:
- Non-isotopic labeling eliminates the hassle of hazardous radioactive materials or difficult-to-dispose-of waste
- 1-3 biotinylated ribonucleotides onto the 3' end of DNA strands for less interference with hybridization or sequence-specific binding of proteins
- Biotin-labeled probes are stable for more than one year
- 30-minute labeling procedure is fast and efficient
Includes:
1mL 5X TdT Reaction Buffer, 50μL Terminal Deoxynucleotidyl Transferase, 100microliter Biotin-11-dUTP, 140μL Unlabeled Control Oligo, 40μL Biotin-Control Oligo
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Specifications
Specifications
Content And Storage | Sufficient For: 20 labeling reactions (each with 5 pmol DNA) • 1 mL 5X TdT Reaction Buffer (-20°C) • 5 μL Terminal Deoxynucleotidyl Tranferase (-20°C) • 1 μL Biotin-11-UTP (≤4°C) • 14 μL Unlabeled Control Oligo (-20°C) • 4 μL Biotin-Control Oligo (-20°C) |
Format | Kit |
Labeling Target | DNA |
No. of Reactions | 20 |
Product Line | Pierce™ |
Product Type | DNA Labeling Kit |
Quantity | 20 Reactions |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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