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Thermo Scientific™ Pierce™ Firefly Luciferase Flash Assay Kit
Description
The Thermo Scientific™ Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.
Highlights:
- Highly sensitive detection of firefly luciferase activity
- Use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer for best results (maximum signal)
- Working solution stable for up to 2 months at -20°C
- Assay reagents compatible with other firefly luciferases
- Amenable to automation
- Contains a universal cell lysis buffer and optimized flash assay reagent
- Safe non-radioactive assay
Includes:
Cell lysis buffer, reaction buffer and substrate
Requires:
Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; For best results (maximum signal), use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer
Recommended for:
Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

Specifications
Specifications
Type | Assay Kit |
Target | Luciferase, Firefly Luciferase |
Quantity | 100 Reaction Kit |
Content And Storage | • Firefly Flash Assay Buffer, 5 mL, store at 4°C • D-Luciferin, Lyophilized, 3 mg, store at 4°C • 2X Cell Lysis Buffer, 6 mL, store at room temperature Upon receipt store kit at 4°C or store individual components as indicated above. |
Assay | Reporter Enzyme, Luciferase Reporter Assay |
Compatible Cells | Mammalian Cells |
Detection Method | Bioluminescence |
For Use With (Equipment) | Luminometer (Microplate) |
Format | 384-well plate, 96-well plate |
Label Type | Enzyme Labeled |
Show More |
For Research Use Only. Not for use in diagnostic procedures.
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