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Thermo Scientific™ Fluoraldehyde™ o-Phthaldialdehyde Crystals (OPA)
Description
Thermo Scientific™ Pierce Fluoraldehyde (OPA) is o-phthalaldehyde, a highly sensitive fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.
Fluoraldehyde Crystals are a specially purified, fluorogenic-grade preparation of o-phthalaldehyde (OPA). This amine-reactive compound is primarily used for high-sensitivity, fluorescent detection of primary amines, including amino acids, peptides, proteins and polyamines in pre- or post-column chromatographic effluents.
Highlights:
For pre- or post-column amino acid derivatization for fluorescent detection and quantitation
Reacts with all primary amine-containing analytes to yield fluorescent derivatives (ex/em = 340nm/455nm)
Provides an accurate measure of both composition and absolute protein-peptide content
Ideal for work with recombinant proteins and synthetic peptides
Can be used for fluorescent protein or peptide assay
Can be prepared as a stable aqueous solution in borate (see instructions)
Reagent Properties:
Can be prepared as a stable aqueous solution in borate (see instructions)
Alternative names: Fluoraldehyde Crystals, OPA
Chemical name: ortho-Phthalaldehyde
Reactive toward: Primary amines (–NH2)
Chemical formula: C8H6O2
CAS number: 643-79-8
Molecular weight: 134.12
Form: Pale yellow, free-flowing crystals

Specifications
Specifications
Product Type | o-Phthaldialdehyde |
Chemical Reactivity | Amine |
Product Line | Fluoraldehyde™ |
Content And Storage | Store at room temperature. |
Label or Dye | OPA (o-phthaldialdehyde) |
Detection Method | Fluorescence |
Quantity | 5 g |
Form | Solid |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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