Thermo Scientific Pierce GST Protein Interaction Pull-Down Kit
Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.
Manufacturer: Thermo Scientific 21516
Purify protein interaction complexes involving practically any user-supplied GST-tagged fusion protein with Thermo Scientific Pierce GST Protein Interaction Pull-Down Kit.The GST Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with recombinant proteins containing a glutathione S-transferase (GST) fusion tag. You provide the tagged fusion protein (bait) and cells expressing the putative protein interaction target (prey), and the pull-down kit supplies everything else: cell lysis buffer, microcentrifuge spin columns, glutathione affinity resin (agarose beads), optimized binding and elution buffers, and a detailed protocol. This kit is designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.
GST pull-down purifies protein interactors of any GST-tagged fusion protein
Complete kit provides all components and detailed protocol for purifying protein:protein interactions
No special equipment needed uses common laboratory equipment and reagents (e.g., microcentrifuge)
Convenient microcentrifuge spin columns facilitate simple and efficient manipulation of glutathione agarose beads, including simple processing of multiple samples
Flexible instructions include protocols for bait and prey proteins from different sources
Glutathione agarose resin, lysis buffer, TBS pack, glutathione for elution, microcentrifuge spin columns and collection tubes
Discover a new protein:protein interaction from a cell lysate; Confirm a putative interaction from a cell lysate or with a previously purified protein; Extract protein:protein interaction information from in vitro transcription/translation lysates
|Sufficient For: At least 25 pull-down reactions, each using 25 µL of resin
• Glutathione Agarose, 750 µL
• Pull-Down Lysis Buffer, 250 mL
• Glutathione, 1 g
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes
Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.