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Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit

Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.

Manufacturer:  Thermo Scientific™ 21516

Catalog No. PI21516


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Description

Description

Purify protein interaction complexes involving practically any user-supplied GST-tagged fusion protein with Thermo Scientific™ Pierce GST Protein Interaction Pull-Down Kit.

The GST Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with recombinant proteins containing a glutathione S-transferase (GST) fusion tag. You provide the tagged fusion protein (bait) and cells expressing the putative protein interaction target (prey), and the pull-down kit supplies everything else: cell lysis buffer, microcentrifuge spin columns, glutathione affinity resin (agarose beads), optimized binding and elution buffers, and a detailed protocol. This kit is designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.

Highlights:

  • GST pull-down – purifies protein interactors of any GST-tagged fusion protein

  • Complete kit – provides all components and detailed protocol for purifying protein:protein interactions

  • No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge)

  • Convenient – microcentrifuge spin columns facilitate simple and efficient manipulation of glutathione agarose beads, including simple processing of multiple samples

  • Flexible – instructions include protocols for bait and prey proteins from different sources

Includes:

Glutathione agarose resin, lysis buffer, TBS pack, glutathione for elution, microcentrifuge spin columns and collection tubes

Recommended for:

Discover a new protein:protein interaction from a cell lysate; Confirm a putative interaction from a cell lysate or with a previously purified protein; Extract protein:protein interaction information from in vitro transcription/translation lysates

Specifications

Specifications

Affinity Purification
Microcentrifuge
Not Target-Specific
Sufficient For: At least 25 pull-down reactions, each using 25 µL of resin
• Glutathione Agarose, 750 µL
• Pull-Down Lysis Buffer, 250 mL
• Glutathione, 1 g
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes

Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
Pierce™
Pull-Down (Tag-Based)
Documents
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