Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit

Catalog No.	Quantity
PI21516	25 reactions kit

Catalog No. PI21516 Supplier Thermo Scientific™ Supplier No. 21516

Description

Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.

Thermo Fisher Scientific Pierce GST Tag Protein Interaction Pull-Down Kits contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.

Features of GST Tag Protein Interaction Pull-Down Kit:

• GST pull-down (Product No. 21516)—gently purifies protein interactors of any GST-tagged fusion protein without denaturing
• Complete kits—provide all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)
• Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
• Flexible—instructions include protocols for use with bait and prey proteins from many different sources
• Binding—Binds approx. 8 mg of GST-tagged fusion protein per mL of resin

Applications:

• Discover a new protein:protein interaction from a cell lysate
• Confirm a putative interaction from a cell lysate or with a previously purified protein
• Extract protein:protein interaction information from in vitro transcription/translation lysates

You provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.

Related Products

Pierce™ His Protein Interaction Pull-Down Kit (Catalog #21277)

Specifications

• Bed Volume: 750 μL
• Capacity (Metric): 1.5 mL
• Formulation: Slurry: 50 %
• Content And Storage: Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin
  • Glutathione Agarose, 750 μL
  • Pull-Down Lysis Buffer, 250 mL
  • Glutathione, 1 g
  • TBS Pack (makes 500 mL), 1 pack
  • Pierce Spin Columns and Accessories, 27 columns
  • Microcentrifuge Collection Tubes, 100 tubes
  
  Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
• Assay: Affinity Purification
• For Use With (Equipment): Microcentrifuge
• Product Line: Pierce
• Quantity: 25 reactions kit
• For Use With (Application): Pull-Down (Tag-Based)
• Target: Not Target-Specific
• Type: Pull-Down Kit
• Sufficient For: 25 Reactions
• Format: Kit

For Research Use Only. Not for use in diagnostic procedures.