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Thermo Scientific™ HABA (4'-hydroxyazobenzene-2-carboxylic acid)

Catalog No. PI28010
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Quantity:
10 g

Description

A colored, biotin assay reagent HABA (4'-hydroxyazobenzene-2-carboxylic acid) to measure biotinylation levels of biotinylated antibodies or proteins.

Thermo Scientific™ Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules.

Thermo Scientific Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules. Determine the molar ratio of biotin incorporated into a protein using the HABA-Avidin method. The HABA dye (4'-hydroxyazobenzene-2-carboxylic acid ) binds to avidin to produce a yellow-orange colored complex which absorbs at 500nm. Free biotin will displace the HABA dye and cause the absorbance to decrease. A standard curve can be established using the free biotin to estimate the number of moles of biotin incorporated after biotinylating a protein.

Highlights:

  • HABA-avidin complex can be used over a wide range of pH and salt concentrations
  • Amount of Avidin can be calculated directly from the increased absorbance at 500nm complexing with the HABA Dye
  • Calculate results directly from absorbance values based on extinction coefficients using the procedure outlined in the instructions or use Pierce's online HABA calculator
  • Complete kits also available! See Pierce Biotin Quantitation Kit (Part No. 28005) and Fluorescence Biotin Quantitation Kit (Part No. 46610)
TRUSTED_SUSTAINABILITY

Specifications

Product Type HABA
Quantity 10 g
Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature.
Product Line Pierce™
Label or Dye Biotin
Labeling Target Antibodies, Proteins

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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