Learn More
Thermo Scientific™ Pierce™ Hydroxylamine-HCl
Description
Thermo Scientific™ Pierce Hydroxylamine-HCl has many uses, including to cleave Asn-Gly peptide bonds and to deprotect (expose) sulfhydryls for conjugation following modification with SATA and similar reagents.
Thermo Scientific™ Pierce Hydroxylamine-HCl has many uses, which include cleaving Asn-Gly peptide bonds and deprotecting (exposing) sulfhydryls on SATA-modified molecules for conjugation. Hydroxylamine·HCl is a strong reducing agent that is useful in biochemical crosslinking applications, including the deacetylation of SATA and chemical cleavage of EGS and Sulfo-EGS. Hydroxylamine converts carbonyl compounds (aldehydes and ketones) to their oxime derivatives in the presence of a weak base. Therefore, crosslinkers and other compounds that contain a carbonyl within their structure are cleavable with hydroxylamine-HCl.
Highlights:
- Exposes protected sulfhydryl groups on SATA-modified molecules
- Cleaves carbonyl-containing crosslinkers such as EGS
Reagent Properties:
- Chemical name: Hydroxylamine Hydrochloride
- Chemical formula: NH2OH·HCl
- Molecular weight: 69.49
- CAS Number: 5470-11-1
- Form: White crystals, free of foreign material
- Solubility: 10%
Specifications
Specifications
| CAS | 5470-11-1 |
| Content And Storage | Upon receipt store desiccated at room temperature. Product shipped at ambient temperature. |
| Product Line | Pierce |
| Form | Solid |
| Reagent Type | SATA |
| Quantity | 25 g |
| Product Type | Hydroxylamine Hydrochloride |
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.