Thermo Scientific™ Immobilized Pepsin (Agarose Resin)

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

Manufacturer:  Thermo Scientific™ 20343

Catalog No. PI20343

Description

Thermo Scientific™ Pierce Immobilized Pepsin consists of an acidic endopeptidase (pepsin) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.

Immobilized pepsin can be substituted for free pepsin in any application, and is advantageous because it virtually eliminates autolysis, eliminates contamination of a sample with the protease and allows control of the digestion by removing the pepsin. Immobilized pepsin is also more stable against heat-induced denaturation, resulting in longer maintenance of activity. The F(ab')2 Preparation Kit contains immobilized pepsin, other necessary reagents and an optimized protocol for human, rabbit and mouse antibody digestion.

Highlights:

• Pepsin can cleave Fc fragments from IgG, resulting in F(ab')₂ fragments
• Broad specificity, including esterase activity
• Easy to use - simply remove the agarose beads to stop the enzymatic digestion

Includes:

Kit contains immobilized pepsin, other necessary reagents and an optimized protocol for human, rabbit and mouse antibody digestion.

Specifications

• Content And Storage: Upon receipt store product at 4-8°C. Product is shipped at ambient temperature.