Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Crosslinking, Labeling and Protein Modification
  5. Protein Modification Reagents
  6. PI77712

Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel

Catalog No. PI77712
Change view
Click to view available options
Quantity:
5 mL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI77712 5 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PI77712 Supplier Thermo Scientific™ Supplier No. 77712
Only null left
Add to Cart
Edge
Add to Cart

Description

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.

Thermo Scientific™ Pierce Immobilized TCEP Disulfide Reducing Gel is Tris(2-carboxyethyl)phosphine that is covalently immobilized to beaded agarose resin to enable efficient reduction of peptide and protein disulfide bonds.

Trialkylphosphines serve as excellent agents for the reduction of disulfide bonds in proteins, peptides and other disulfide bond-containing molecules and are relatively unreactive toward other functional groups. The trialkylphosphine TCEP is stable in aqueous solutions and does not undergo the rapid oxidation that often occurs with other reducing agents such as dithiotreitol (DTT) and β-mercaptoethanol (BME). TCEP does not interfere with common sulfhydryl-reactive reagents (e.g., maleimide crosslinkers). Nevertheless, many protocols require recovery of the reduced sample separate from the reducing agent. Immobilized TCEP Disulfide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent. Immobilized TCEP Disulfide Reducing Gel may be adapted conveniently to a variety of scales and formats (i.e. batch, spin column and gravity-flow column procedures).

Highlights:

  • Odorless: reduce proteins at your desktop; contributes to a healthier lab environment
  • Stable: virtually no loss of activity when stored refrigerated
  • Versatile: reduces peptides and proteins over a broad range of pH, salt, detergent and temperature conditions
  • Flexible: resin slurry provides flexibility for use in spin tube, column or batch modes
  • Time-saving: no soluble reductant removal saves time and conserves sample
  • Efficient: reverses both natural disulfide bonds and disulfide bonds artificially incorporated by crosslinking; excellent alternative for uncoupling crosslinked proteins or peptides by agents such as DSP and DTSSP
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Upon receipt store at 4°C. Do not freeze.
Product Line Pierce
Form Liquid
Quantity 5 mL
Product Type Disulfide Reducing Reagent

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.