Thermo Scientific™ Pierce™ Iodoacetic Acid

Description

Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.

Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.

Iodoacetic Acid (IAA) can react with several protein functional groups but is typically used for specific S-carboxymethylation of sulfhydryls (reduced cysteines).

Iodoacetic acid reacts with sulfhydryls on cysteines, imidazolyl side chain nitrogens of histidines, the thioether of methionine and the primary amine group of lysines.The rate of reaction and specificity is dependent on the ionization level, which can be manipulated by the pH of the reaction condition.

Highlights:

• React at slightly alkaline pH for specific S-carboxymethylation of free sulfhydryls
• React at low pH for specific carboxymethylation of methionines
• React at high pH to favor carboxymethylation of histidines and lysines
• Methylate reduced cysteine peptide fragments in protease digests for mass spectrometry

Specifications

• Chemical Reactivity: Thiol
• Description: Iodoacetic Acid
• Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
• Label or Dye: Unreactive Group
• Label Type: Blocking Groups
• Product Line: Pierce™
• Spacer: Short (<10 Å)
• Form: Powder
• Quantity: 500 mg
• Solubility: Water
• Product Type: Iodoacetic Acid

For Research Use Only. Not for use in diagnostic procedures.