Thermo Scientific™ Pierce™ Kinase Enrichment Kit with ATP Probe

Description

Desthiobiotin-ATP and -ADP probes to label, affinity purify and measure the active site of ATPases (kinases), including chaperones and metabolic enzymes.

Thermo Scientific™ Pierce Kinase Enrichment Kits utilize ActivX™ ATP or ADP Probes to covalently label the active site of ATPases, including chaperones and metabolic enzymes, to enable their selective enrichment using a desthiobiotin tag.

These products, based on ActivX ATP and ADP Probes, feature an amine-reactive nucleotide analog and a desthiobiotin (biotin analog) tag that facilitates selective labeling of lysines in the kinase active site and then subsequent enrichment and recovery of labeled protein. These features allow identification and profiling of target enzyme classes across samples or assessment of the specificity and affinity of enzyme inhibitors.

Highlights:

• Specific – label only the conserved active-site lysines of nucleotide-binding proteins

• Flexible – use for in vitro labeling of ATPase enzymes derived from cells or tissues

• Compatible – use with Western blot or mass spectrometry (MS) workflows

Recommended for:

Profile small-molecule binding affinities and active-site inhibition in a dose-dependent manner; Identify dozens to hundreds of inhibitor targets and off-targets from tissues, cells and subcellular proteomes; Enrichment of enzymes based on function.

Specifications

• Formulation: Multi-component kit with Desthiobiotin-ATP Probe
• Content And Storage: ActivX Desthiobiotin-ATP Probe is shipped separately with dry ice and stored at -80°C upon receipt. All other kit components are shipped at ambient temperature and stored at 4°C upon receipt.
• Assay: Protein Quantitation Assay
• Detection Method: Chemiluminescence, Mass Spectrometry, Western Blot
• Substrate Type: Kinase Substrate
• Product Line: ActivX™, Pierce™
• Quantity: 16 reactions
• For Use With (Application): Pull-Down
• Target: Kinases (Multiple), Phosphodiesterases, ATPases, GTPases, Phosphatases
• Type: Kinase Enrichment Kit
• Label or Dye: Biotin & Other Hapten
• Format: Tube, Spin Column

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.