Thermo Scientific™ Pierce™ Magnetic RNA-Protein Pull-Down Kit

Description

Utilize a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait with Thermo Scientific™ Pierce Magnetic RNA-Protein Pull-Down Kit.

Utilize a streamlined, robust method to enrich for protein-RNA interactions using end-labeled RNA as the bait with Thermo Scientific™ Pierce Magnetic RNA-Protein Pull-Down Kit.

Additional Information and Online Ordering

The Magnetic RNA-Protein Pull-Down Kit provides reagents to efficiently enrich RNA Binding Proteins (RBPs) using RNA end-labeled with desthiobiotin and streptavidin magnetic beads. The complete kit contains sufficient reagents for 20 RNA labeling reactions and 20 protein-RNA pull-down assays. This direct enrichment of the protein-RNA interaction provides an alternative to antibody capture of protein-RNA complexes or moieties incorporated into the nucleic acid. An added advantage to the kit is that it includes validated controls for both the labeling and pull-down assay. The kit is amenable to several downstream applications, including Western blotting and Mass Spectrometry (MS).

Highlights:

• Direct – capture ribonucleoprotein complexes directly with end-labeled RNA; does not use or require antibodies for pull-down
• Easy to use – no spin cups or centrifugation necessary for the enrichment of RBP; procedure streamlined for minimal hands-on time (less than 3 hours) after RNA labeling reaction
• Flexible – use in vitro transcribed RNA or synthetic RNA for labeling of various lengths and complexity; proteins successfully enriched using endogenous, over-expressed, and in vitro translated lysates
• Specific – low bead background; unrelated RNA or mutated RNA does not significantly enrich specified RBPs
• Economical – less expensive than purchasing commercially synthesized end-labeled RNA, magnetic beads, and reagents separately
• Complete – contains both labeling and enrichment modules with buffers necessary for assay; positive control RNA, negative control RNA, and RBP antibody included

Includes:

Complete kit contains the Pierce RNA 3'-End Desthiobiotinylation Kit, positive and negative RNA controls, nucleic-acid compatible streptavidin magnetic beads, and buffers for RBP enrichment and elution

Requires:

User-supplied RNA and lysate (experimental sample)

Recommended for:

• Mutational analysis
• Structure function analysis
• Identification of RNA:Protein interactions
• MS analysis of unknown RNA:protein binding pairs or complexes

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Specifications

• Capacity (Metric): 1 mL
• Kit Contents: 50 pmol of RNA per Reaction
• Content And Storage: Sufficient For: 20 RNA-protein complex pull-down reactions
  
  Slurry: 10mg/mL, 1% solids
  • Pierce Nucleic Acid-Compatible Streptavidin Magnetic Beads, 1 mL (store at 4°C)
  • RNA Capture Buffer (1X), 10 mL (store at 4°C)
  • Tris (20 mM, pH 7.5), 5 mL (store at 4°C)
  • Protein-RNA Binding Buffer (10X), 1 mL (store at 4°C)
  • Wash Buffer (1X), 10 mL (store at 4°C)
  • Biotin Elution Buffer, 1.5 mL (store at 4°C)
  • HuR Monoclonal Antibody (Mouse), 50 μL (store at 4°C)
  • Positive RNA Control (AR RNA), 250 pmol (store at -20°C)
  • Negative RNA Control (polyA₂₅ RNA), 250 pmol (store at -20°C)
  • Pierce RNA 3' End Desthiobiotinylation Kit (part number 20163; store at -20°C)
• Product Line: Pierce™
• Quantity: 20 reactions kit
• Type: RNA-Protein Pull Down Kit
• Sufficient For: 20 Reactions
• Format: Kit

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.