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Thermo Scientific™ EZ-Link™ Maleimide Activated Horseradish Peroxidase Kit

Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.

Supplier:  Thermo Scientific™ 31494

Catalog No. PI31494


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Description

Description

Thermo Scientific™ Pierce Maleimide-Activated HRP Conjugation Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

The Thermo Scientific Maleimide Activated Horseradish Peroxidase (HRP) Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain a free sulfhydryl group. This kit eliminates the first step of the two-step maleimide method, making conjugate preparation much simpler. The kit is supplied with a stable, Maleimide-activated horseradish peroxidase. HRP conjugates can be purified by gel filtration chromatography, ultrafiltration or dialysis, depending upon the size of the conjugated protein.

Highlights:

  • Prepare enzyme conjugates from proteins that contain a free sulfhydryl
  • Includes 2-MEA to generate free sulfhydryls from disulfide bonds
  • Includes SATA to add free sulfhydryls to lysine residues
Specifications

Specifications

1 Enzyme Labeling Reaction
EZ-Link™
Thiol
HRP (Horseradish Peroxidase)
Maleimide
1 Reaction
Sufficient For: 1 enzyme-labeling reaction with up to 5 mg of antibody (IgG)
• Maleimide Activated Horseradish Peroxidase, 5 mg
• Conjugation Buffer (10X), 20 mL
• PBS Pack (makes 500mL), 1 pack
• 2-Mercaptoethylamine-HCl, 6 mg
• SATA, 2 mg
• Dimethylformamide, 1 mL
• Hydroxylamine-HCl, 5 mg
• Polyacrylamide Desalting Column, 10 mL, 1 column

Store at 4°C.
Enzyme Labeled
Water
Multi-component kit with activated HRP
SDS
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