Thermo Scientific™ Pierce™ Phosphoprotein Enrichment Kit

Description

Purify (enrich) phosphorylated proteins from mammalian cell and tissue lysates using phospho-specific IMAC affinity resin cartidges or kit.

Perform efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues with Thermo Scientific™ Pierce Phosphoprotein Enrichment Kit.

The kit is an effective system for isolating the phosphoprotein fraction of mammalian cell and tissue lysates. The included lysis-binding buffer and proprietary affinity resin produce superior yields with negligible non-specific binding. The kit uses a convenient spin column format, and the resulting phosphorylated protein sample is compatible with downstream applications, including Western blotting, mass spectrometry (MS), 2D-PAGE, and protein arrays. The affinity resin is also available as pre-packed chromatography cartridges for use with automated liquid chromatography (LC) systems.

Highlights:

• Specific – low nonspecific protein contamination from complex biological samples, such as cell culture lysate and mouse tissue extract

• Fast – easy spin format enables enrichment of phosphorylated proteins in less than 2 hours

• Superior yield – achieves higher yields than other commercially available phosphoprotein isolation kits

• Convenient format – complete kit offering pre-dispensed spin columns, buffers, reagents and ultrafiltration columns for enrichment

• Compatible – downstream applications include mass spectrometry, Western blotting and 2D-PAGE

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Specifications

• Content And Storage: Upon receipt store at 4°C.
• Product Line: Pierce™
• Quantity: 10 Column Kit
• Target: Phosphoprotein
• Type: Phosphoprotein Enrichment Kit
• Stationary Phase: Proprietary Ligand
• Sufficient For: 10 Reactions
• Format: Spin Column
• Column Type: Affinity, Agarose Resin

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.