Thermo Scientific™ Pierce™ Phosphoprotein Enrichment Kit

Purify (enrich) phosphorylated proteins from mammalian cell and tissue lysates using phospho-specific IMAC affinity resin cartidges or kit.

Supplier:  Thermo Scientific™ 90003

Catalog No. PI90003

Description

Perform efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues with Thermo Scientific™ Pierce Phosphoprotein Enrichment Kit.

The kit is an effective system for isolating the phosphoprotein fraction of mammalian cell and tissue lysates. The included lysis-binding buffer and proprietary affinity resin produce superior yields with negligible non-specific binding. The kit uses a convenient spin column format, and the resulting phosphorylated protein sample is compatible with downstream applications, including Western blotting, mass spectrometry (MS), 2D-PAGE, and protein arrays. The affinity resin is also available as pre-packed chromatography cartridges for use with automated liquid chromatography (LC) systems.

Highlights:

• Specific – low nonspecific protein contamination from complex biological samples, such as cell culture lysate and mouse tissue extract

• Fast – easy spin format enables enrichment of phosphorylated proteins in less than 2 hours

• Superior yield – achieves higher yields than other commercially available phosphoprotein isolation kits

• Convenient format – complete kit offering pre-dispensed spin columns, buffers, reagents and ultrafiltration columns for enrichment

• Compatible – downstream applications include mass spectrometry, Western blotting and 2D-PAGE

Specifications

• Content And Storage: Upon receipt store at 4°C.
• Quantity: 10 Column Kit
• Type: Phosphoprotein Enrichment Kit
• Sufficient For: 10 Reactions
• Column Type: Affinity, Agarose Resin

• Product Line: Pierce™
• Target: Phosphoprotein
• Stationary Phase: Proprietary Ligand
• Format: Spin Column

For Research Use Only. Not for use in diagnostic procedures.