Thermo Scientific™ Pierce™ His Protein Interaction Pull-Down Kit

Description

Easily capture and affinity purify binding partners of His-tagged fusion proteins using nickel-chelate (Ni-IDA) agarose beads and this complete reagent set.

Thermo Scientific™ Pierce PolyHis Protein Interaction Pull-Down Kit purifies protein interaction complexes involving practically any user-supplied His-tagged fusion protein.

The PolyHis Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with recombinant proteins containing a histidine (6xHis) fusion tag. You provide the tagged fusion protein (bait) and cells expressing the putative protein interaction target (prey), and the pull-down kit supplies everything else: cell lysis buffer, microcentrifuge spin columns, cobalt-IMAC affinity resin (agarose beads), optimized binding and elution buffers, and a detailed protocol. This kit is designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.

Highlights:

• 6xHis pull-down – purifies protein interactors of any His-tagged fusion protein

• Complete kit – provides all components and detailed protocol for purifying protein:protein interactions

• No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge)

• Convenient – microcentrifuge spin columns facilitate simple and efficient manipulation of cobalt IMAC agarose beads, including simple processing of multiple samples

• Flexible – instructions include protocols for bait and prey proteins from different sources

Includes:

Cobalt chelate agarose resin, lysis buffer, TBS pack, imidazole solution for elution, microcentrifuge spin columns and collection tubes

Recommended for:

Discover a new protein:protein interaction from a cell lysate; Confirm a putative interaction from a cell lysate or with a previously purified protein; Extract protein:protein interaction information from in vitro transcription/translation lysates

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Specifications

• Content And Storage: Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin
  • Cobalt Chelate Agarose Resin, 750 μL
  • Pull-Down Lysis Buffer, 250 mL
  • Imidazole Solution (4M), 5 mL
  • TBS Pack (makes 500 mL), 1 pack
  • Pierce Spin Columns and Accessories, 27 columns
  • Microcentrifuge Collection Tubes, 100 tubes
  
  Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
• Assay: Affinity Purification
• For Use With (Equipment): Microcentrifuge
• Product Line: Pierce™
• Quantity: 25 reactions kit
• For Use With (Application): Pull-Down (Tag-Based)
• Target: Not Target-Specific
• Type: Pull-Down Kit
• Sufficient For: 25 Reactions
• Format: Kit

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.