Thermo Scientific™ Pierce™ Colorimetric Protease Assay Kit

Catalog No.	Quantity
PI23263	250 Test Kit

Catalog No. PI23263 Supplier Thermo Scientific™ Supplier No. 23263

Description

Determine total protease activity in biological samples via fluorescent or colorimetric detection by measuring digestion of labeled casein substrate.

The Thermo Scientific Pierce Colorimetric Protease Assay Kit measures total protease activity in samples, providing a means to assess the progress of protease isolation procedures or quantify protease contamination in biological samples. This kit uses fully succinylated casein as a substrate for the assay. Hydrolysis of this substrate in the presence of protease results in the release of peptide fragments with free amino-terminal groups. These peptides are reacted with trinitrobenzene sulfonic acid (TNBSA), followed by measurement of the absorbance increase that results from the formation of yellow colored TNB-peptide adducts.

Features of the Colorimetric Protease Assay Kit:

• Casein substrate—measure activity of any protease that cleaves casein into peptide fragments
• Trypsin standard—quantify protease activity relative to trypsin, a universally accepted reference
• Sensitive—1000 times more sensitive than assays that use unmodified forms of casein
• Simple and fast—test tube and microplate protocols are completed in less than one hour
• Homogenous—addition steps only; no separation, transfer or stop steps required
• Customizable—easily adapt time, temperature and pH to optimize sensitivity for proteases of interest

Applications:

• Assess functional integrity and overall progress of protease purification
• Quantify protease contamination in protein samples
• Characterize the activity of unusual proteases compared to trypsin
• Evaluate buffer conditions for their affects on protease function and activity

Additional Specifications:

• Substrate: succinylated casein (not available separately)
• Basis of assay: detection of primary amines resulting from proteolysis
• Detection reagent: trinitrobenzene sulfonic acid (TNBSA)
• Measurement: absorbance at 450nm (plate reader or spectrophotometer)

Related Products

Pierce™ Fluorescent Protease Assay Kit (Cat. No. 23266)

FITC-Casein for Pierce™ Fluorescent Protease Assay Kit (Cat. No. 23267)

Specifications

• Content And Storage: Sufficient For: 250 microplate assays using a standard plate reader (absorbance at 450 nm)
  • Succinylated Casein, 5 x 10 mg
  • TNBSA Solution, 2 mL
  • TPCK Trypsin Standard, 50 mg
  • Borate Buffer Pack (makes 500 mL), 1 pack
  
  Store at 4°C.
• Assay: Protease Assay
• Detection Method: Absorbance
• Immunoassay Kit Format: 96-well Plate
• Product Line: Pierce
• Product Type: Protein Activity Assay
• Substrate: Casein
• Substrate Properties: Protein-Based Substrate
• Substrate Type: Protease Substrate
• Sufficient For: 250 Microplate Assays
• For Use With (Application): Absorbance
• For Use With (Equipment): Microplate Reader
• Quantity: 250 Test Kit
• Target: Proteases

For Research Use Only. Not for use in diagnostic procedures.