Thermo Scientific™ Pierce™ Protein A/G Plus Agarose

Catalog No.	Quantity
PI20424	20 mL
PI20423	2 mL

Catalog No. PI20424 Supplier Thermo Scientific™ Supplier No. 20424

Description

Thermo Scientific Pierce Protein A/G Plus Agarose is an exceptionally high-capacity Protein A/G beaded agarose resin for use in a variety of antibody affinity purification methods.

Pierce Protein A/G Plus Agarose consists of purified Protein A/G recombinant fusion protein that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose (CL-6B). Because the Protein A/G combines the immunoglobulin-binding domains of both Protein A and Protein G, the resin is effective for affinity purification of IgG antibodies from a broad range mammalian host species.

Highlights:

• Protein A/G – immobilized recombinant fusion protein of the antibody-binding domains of Protein A and Protein G enables polyclonal IgG purification from nearly any mammalian species
• Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
• Inert and stable – superior manufacturing method immobilizes Protein A/G by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
• High capacity – “Plus” variety of Pierce Protein A/G Agarose has a very dense load of immobilized Protein A/G, providing a binding capacity greater than 50mg human IgG/mL resin

Properties of crosslinked 6% beaded agarose (CL-6B):

• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1mL/minute (for 1cm diameter column)
• Maximum Linear Velocity: 30cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

Specifications

• Product Type: Agarose
• Stationary Phase: Protein A/G
• Purification Target: IgG Antibodies, Ig Antibodies
• For Use With (Application): Antibody Purification
• Content And Storage: Protein A/G, Agarose Resin. Store in refrigerator (2–8°C).
• Quantity: 20 mL
• Shipping Condition: Wet Ice
• Form: Agarose Bead

For Research Use Only. Not for use in diagnostic procedures.