The Thermo Scientific Pierce Protein A/G Magnetic Beads are high-performance affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.These magnetic beads are coated with genetically engineered Pierce Protein A/G, a recombinant fusion protein which combines the IgG binding domains of both Protein A and Protein G. This enables capture of antibodies from a wider range of species and isotypes than either protein alone. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in your immunoprecipitated sample. These beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments.
High capacity nearly four times higher binding capacity than typical magnetic beads from other suppliers, allowing the use of smaller amounts per experiment
Low non-specific binding stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with antibody is devoid of contaminating proteins from complex IP matrix)
Flexibility convenience of IgG binding domains of both Protein A and Protein G on one bead
Compatibility - beads are compatible with manual and automated applications (e.g., Thermo Scientific KingFisher Instruments)
Assay consistency magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes
Properties of Thermo Scientific Pierce Protein A/G Magnetic Beads:
- Composition: Magnetite-coated polymeric beads blocked and covalently coated with a monolayer of recombinant Protein A/G
- Diameter: 1µm
- Concentration: 10mg/mL
- Binding Capacity: 55-85µg rabbit IgG per mg magnetic particles
Thermo Scientific KingFisher 96 or KingFisher Flex Instrument or other magnetic separator device
IP and Co-IP experiments (see complete kit); Immunoprecipitation for analysis in non-reducing conditions; Antibody purification
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