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Thermo Scientific™ Pierce™ Protein G UltraLink™ Columns, 2 mL

Catalog No. PI53127
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Quantity:
2 Columns

Description

Extremely durable polyacrylamide support with immobilized, recombinant Protein G for various IgG purification methods.

Thermo Scientific™ Pierce Protein G UltraLink Resin is an extremely durable beaded polyacrylamide support to which recombinant Protein G has been immobilized for use in IgG purification methods.

The Protein G UltraLink Resin is used to bind and purify IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein G is a popular antibody-binding protein that is especially useful for affinity purification of IgG from mouse, human and rat serum.

UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.

Highlights:

  • Immobilized Protein G – specifically binds IgG from several mammalian species, especially all isotypes of IgG from human, mouse and rat

  • Standard capacity – loading of immobilized Protein G provides a binding capacity of approx. 20mg human IgG per milliliter of resin

  • UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications

Properties of Thermo Scientific UltraLink Resin:

  • Support pH Stability: 1 to 13
  • Average Particle Size: 50 to 80 microns
  • Exclusion Limit: greater than 2,000,000 daltons
  • Average Surface Area: greater than 250m2 per gram of beads
  • Average Pore Volume: greater than 1.2mL per gram of beads (> 60% of bead volume)
  • Pore Size: 500 to 1000 angstroms (50 to 100nm)
  • Maximum Linear Velocity: 3000cm per hour
  • Maximum Pressure: 100psi (6.9 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)
TRUSTED_SUSTAINABILITY

Specifications

Product Type Spin Column
Product Line Pierce™, UltraLink™
Column Type UltraLink Affinity Resin
Format 15 mL Conical Spin Column
Stationary Phase Protein G
Purification Target IgG Antibodies
Description Pierce Protein G UltraLink Columns
Content And Storage Upon receipt store product at 4°C. Products are shipped at ambient temperature.
Quantity 2 Columns

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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