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Thermo Scientific™ Pierce™ Renilla Luciferase Flash Assay Kit
Description
The Thermo Scientific™ Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.
Highlights:
Sensitive – greater sensitivity allows utilization of smaller numbers of cells
Cost effective – highest sensitivity assays result in decreased reagent consumption
Time-saving – assays require minimal sample handling
Automation-friendly – amenable to high throughput screens
Convenient – contains a universal cell lysis buffer and optimized flash assay reagent
Safe – allows one to perform non-radioactive assays
Includes:
Cell lysis buffer, reaction buffer and substrate
Requires:
Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers
Recommended for:
Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

Specifications
Specifications
Type | Assay Kit |
Target | Luciferase, Green Renilla Luciferase |
Quantity | 1000 Reaction Kit |
Content And Storage | • Renilla Flash Assay Buffer, 50 mL, store at 4°C • Coelenterazine (100X), 0.5 mL, store at -80°C • 2X Cell Lysis Buffer, 60 mL, store at room temperature |
Assay | Reporter Enzyme, Luciferase Reporter Assay |
Compatible Cells | Mammalian Cells |
Detection Method | Bioluminescence |
For Use With (Equipment) | Luminometer (Microplate) |
Format | 384-well plate, 96-well plate |
Label Type | Enzyme Labeled |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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