Thermo Scientific™ Pierce™ RNA 3' End Biotinylation Kit 

Biotinylate 3-prime terminal ends of RNA probes for non-radioactive EMSA and other nucleic acid detection methods.

Manufacturer:  Thermo Scientific™ 20160

Catalog No. 20-160-MI

Description

Thermo Scientific™ Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.

The complete kit contains an unlabeled RNA strand to use as a positive control, a biotinylated-RNA probe standard, RNase inhibitor, glycogen and ligation-enhancing reagents. The RNA 3' End Biotinylation Kit uses T4 RNA ligase to conjugate a single nucleotide analog to the 3' terminus of an RNA strand. The unique biotinylated cytidine (bis)phosphate used in this kit enables T4 RNA ligase to attach a single biotin tag on RNA probes of diverse secondary structure and lengths with greater than 70% efficiency.

Highlights:

• Non-radioactive – incorporates a biotin label which allows detection sensitivity similar to radioactivity when used with chemiluminescent detection systems

• Fast – RNA can be labeled in 0.5-2 hours with minimal downstream processing

• Easy to use – RNA ligase and optimized reaction buffer are included

• Economical – only a fraction of the cost of purchasing synthetic biotinylated RNA probes

• End-labeled – results in minimal disturbance of RNA secondary structure

• Flexible – label synthetic or in vitro transcribed RNA probes ranging from 22 to 450 nucleotides

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Specifications

• Content And Storage: Sufficient For: 20 labeling reactions (each with 50 pmol RNA)
  • T4 RNA Ligase Reaction Buffer (10X), 100 µL
  • T4 RNA Ligase (20,000 U/mL), 40 µL
  • Non-labeled RNA Control (10 µM), 100 µL
  • Biotinylated IRE RNA Control (125 nM), 35 µL
  • RNase Inhibitor (40 U/µL), 2 × 10 µL
  • Nuclease-free Water, 1.5 mL
  • DMSO, 200 µL
  • 30% PEG, 300 µL
  • Biotinylated Cytidine (Bis)phosphate (1mM), 40 µL
  • Glycogen (20 mg/mL), 20 µL
  
  Store all components at -20°C
• Product Line: Pierce™