Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ RNA 3' End Desthiobiotinylation Kit

Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.

Supplier:  Thermo Scientific™ 20163

Catalog No. PI20163

Add to Cart



Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins with Thermo Scientific™ Pierce RNA 3' End Desthiobiotinylation Kit.

Additional Information and Online Ordering »

The Pierce RNA 3'-End Desthiobiotinylation Kit is optimized for labeling the 3'-end of single-stranded RNA using T4 RNA ligase. Once labeled, the RNA can be used as a probe or target for gel-shift EMSA reactions, Northern blots and protein-RNA interaction experiments. The desthiobiotinylated RNA also can be used to enrich for RNA binding proteins (RBP) using streptavidin affinity resin, because the desthiobiotin tag binds to streptavidin in a manner which allows gentle elution of the ribonucleoprotein complex. The labeling kit is also included as a component of the Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit.


  • Non-radioactive

    – a single biotin labels has similar detection sensitivity as radioactivity

  • Easy to use – RNA ligase and optimized reaction buffer are included

  • Economical – only a fraction of the cost purchasing synthetic biotinylated RNA probes

  • End-labeled – results in minimal disturbance of RNA secondary structure

  • Flexible

    – desthiobiotin label may be used for detection or as an affinity handle for streptavidin resin


  • Desthiobiotinylated cytidine bisphosphate, T4 RNA ligase and buffer, unlabeled RNA oligonucleotide for use as a positive control, a biotinylated RNA probe standard, RNase inhibitor, glycogen, and ligation enhancing reagents

Recommended for:

  • Northern blotting
  • EMSA
  • Enrichment of RNA binding proteins (RBP)


Sufficient For: 20 desthiobiotinylation reactions using 50 pmol of RNA each
• Desthiobiotinylated Cytidine Bisphosphate, 40 µL
• T4 RNA Ligase, 40 µL
• T4 RNA Ligase Reaction buffer (10X), 100 µL
• Non-labeled RNA Control, 100 µL
• Biotinylated IRE RNA Control, 35 µL
• RNase Inhibitor, 2 x 10 µL
• DMSO, 200 µL
• PEG (30%), 300 µL
• Glycogen, 20 µL
• Nuclease-free Water, 1.5 mL

Upon receipt, store at -20°C.
Magnetic Bead
20 Reactions
RNA Labeling Kit


Product Certifications


Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.