Thermo Scientific™ Pierce™ RNA 3' End Desthiobiotinylation Kit

Description

Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.

Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins with Thermo Scientific™ Pierce RNA 3' End Desthiobiotinylation Kit.

Additional Information and Online Ordering

The Pierce RNA 3'-End Desthiobiotinylation Kit is optimized for labeling the 3'-end of single-stranded RNA using T4 RNA ligase. Once labeled, the RNA can be used as a probe or target for gel-shift EMSA reactions, Northern blots and protein-RNA interaction experiments. The desthiobiotinylated RNA also can be used to enrich for RNA binding proteins (RBP) using streptavidin affinity resin, because the desthiobiotin tag binds to streptavidin in a manner which allows gentle elution of the ribonucleoprotein complex. The labeling kit is also included as a component of the Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit.

Highlights:

• Non-radioactive

  – a single biotin labels has similar detection sensitivity as radioactivity

• Easy to use – RNA ligase and optimized reaction buffer are included

• Economical – only a fraction of the cost purchasing synthetic biotinylated RNA probes

• End-labeled – results in minimal disturbance of RNA secondary structure

• Flexible

  – desthiobiotin label may be used for detection or as an affinity handle for streptavidin resin

Includes:

• Desthiobiotinylated cytidine bisphosphate, T4 RNA ligase and buffer, unlabeled RNA oligonucleotide for use as a positive control, a biotinylated RNA probe standard, RNase inhibitor, glycogen, and ligation enhancing reagents

Recommended for:

• Northern blotting
• EMSA
• Enrichment of RNA binding proteins (RBP)

Specifications

• Content And Storage: Sufficient For: 20 desthiobiotinylation reactions using 50 pmol of RNA each
  • Desthiobiotinylated Cytidine Bisphosphate, 40 μL
  • T4 RNA Ligase, 40 μL
  • T4 RNA Ligase Reaction buffer (10X), 100 μL
  • Non-labeled RNA Control, 100 μL
  • Biotinylated IRE RNA Control, 35 μL
  • RNase Inhibitor, 2 x 10 μL
  • DMSO, 200 μL
  • PEG (30%), 300 μL
  • Glycogen, 20 μL
  • Nuclease-free Water, 1.5 mL
  
  Upon receipt, store at -20°C.
• Format: Kit
• Isolation Technology: Magnetic Bead
• No. of Reactions: 20
• Product Line: Pierce™
• Product Type: RNA Labeling Kit
• Quantity: 20 Reactions

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.