Accelerate throughput for automated magnetic purification of biotinylated molecules with Thermo Scientific™ Pierce Streptavidin Magnetic Beads.
Magnetic streptavidin beads enable affinity purification of biotin-labeled target molecules without columns or centrifugation. The platform provides a fast and convenient method for manual or automated immunoprecipitation, protein interaction analysis, DNA-protein pull-down, and purification involving biotin-labeled proteins and nucleic acids. The iron oxide superparamagnetic particles offer superior performance (high capacity and low nonspecific binding) compared with other commercial magnetic beads. Biotinylated molecules are simply added to the streptavidin-coated magnetic beads for binding. Pierce Streptavidin Magnetic Beads are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher™ 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand.
High-performance beads – non-aggregating, pre-blocked, iron oxide, superparamagnetic microparticles provide exceptional uniformity for automated HTS and manual applications alike
Stable immobilization chemistry – streptavidin is immobilized using leach-resistant chemistry
High capacity – superior quality beads with high binding capacity provide rapid and efficient biomolecule purification from complex samples
Low non-specific binding – stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with biotinylated antibody) that are compatible with mass spectrometry analysis and other downstream analyses
Superior performance – nearly three times higher binding capacity than typical beads from other suppliers, allowing the use of smaller amounts per experiment
Properties of Streptavidin Magnetic Beads:
- Composition: ion oxide particles covalently coated with a monolayer of streptavidin protein
- Magnetization: superparamagnetic (no magnetic memory)
- Bead size: 1μm (nominal mean diameter)
- Density: 2g/cm3
- Concentration: 10mg beads per milliliter of slurry in 0.05% sodium azide
- Binding capacity: approx. 55μg biotinylated rabbit IgG per mg of beads; approx. 3500pmol biotinylated fluorescein per milliliter of beads
- RNase: these bead solutions are NOT tested and certified to be RNAse-free
Thermo Scientific KingFisher 96 or KingFisher Flex Instrument or other magnetic separator device
Immunoprecipitate antigens (using biotinylated antibodies) from a wide variety of sources; Co-immunoprecipitate interaction complexes using biotinylated antibodies; Capture protein-protein interactions in pull-down assays using biotinylated bait proteins; Isolate biotin-labeled DNA-protein complexes from cell or tissue extracts; Capture single-stranded biotinylated DNA oligos; Isolate biotinylated PCR products
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