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Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit
Description
Thermo Scientific™ Pierce Sulfhydryl Addition Kit is a complete set of reagents, including SATA, to enable modification of primary amines to free sulfhydryl groups for use in heterobifunctional crosslinking techniques.
Highlights:
- Reacts with primary amines to add protected sulfhydryls
- An easy deprotection step generates a free sulfhydryl group
- Thermo Scientific™ Sulfhydryl Addition Kit contains all necessary components for thiolation, deprotection, purification and quantitation
Specifications
Specifications
| Chemical Reactivity | Amine |
| Content And Storage | Sufficient For: One modification experiment with 0.5 to 5 mg of purified protein • SATA, 2 mg • Hydroxylamine·HCl, 5 mg • Conjugation Buffer Stock (10X), 20 mL • PBS pack (makes 500 mL), 1 pack • Dimethylformamide (DMF), 1 mL • Dextran Desalting Column, 1 x 5 mL • Column Extender, 1 • Ellman's Reagent (DTNB), 2 mg • Cysteine·HCl, 20 mg Store at 4°C. |
| Label or Dye | Sulfhydryl Group |
| Label Type | Conjugating Groups |
| Product Line | Pierce |
| Crosslinker Type | Heterobifunctional |
| Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
| Spacer | Short (<10 Å) |
| Form | Powder |
| Reagent Type | SATA |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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