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Thermo Scientific™ Sulfo-SBED Biotin Label Transfer Kit - Western Blot Application
Description
Thermo Scientific™ Pierce Sulfo-SBED Biotin Label Transfer WB Kit includes Sulfo-SBED Reagent to crosslink interacting proteins and transfer a biotin label from starting protein to target protein for detection by Western blotting.
Highlights:
- Sulfo-NHS ester reacts with primary amines at pH 7-9
- Aryl azide conjugates randomly when activated with UV light
- Cleavable disulfide bond allows biotin label transfer
- Discover protein interactions without radiolabeling
Includes:
Sulfo-SBED No-Weigh™ Format 8 x 1mg microtubes, BupH™ Phosphate Buffered Saline 1 pack, Label Transfer Buffer (20X) 200mL, Streptavidin-Horseradish Peroxidase Conjugate 0.1mg, Dithiothreitol (DTT) No-Weigh Format 8 x 7.7mg microtubes, Slide-A-Lyzer™ MINI Dialysis Units Plus Float 10kDa MWCO 10 units/pack

Specifications
Specifications
Format | Kit |
Quantity | 8 Reactions |
Product Type | Crosslinker |
Solubility | Water |
Content And Storage | Sufficient For: 8 label transfer reactions for subsequent western blot analysis • Sulfo-SBED, 8 x 1 mg • PBS pack (makes 500 mL), 1 pack • Label Transfer Buffer (20X), 200 mL • Streptavidin-HRP Conjugate, 0.1 mg • Dithiothreitol (DTT), 8 x 7.7 mg • Slide-A-Lyzer MINI Dialysis Devices Plus Float, 10K MWCO, 10-device kit Store at 4°C |
Labeling Method | Chemical Labeling, Label Transfer |
Reactive Moiety | Sulfo-NHS Ester, Aryl Azide |
Product Line | Pierce™ |
For Research Use Only. Not for use in diagnostic procedures.
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