Thermo Scientific™ Sulfo-SBED Biotin Label Transfer Kit - Western Blot Application

Description

Tag a purified protein to covalently crosslink and transfer biotinylation to its binding partner; then detect the results by Western blotting.

Thermo Scientific™ Pierce Sulfo-SBED Biotin Label Transfer WB Kit includes Sulfo-SBED Reagent to crosslink interacting proteins and transfer a biotin label from starting protein to target protein for detection by Western blotting.

Label transfer is a powerful in vitro method for protein interaction discovery. Sulfo-SBED Biotin Label Transfer Reagent can be used to identify previously unknown protein interaction binding partners and to more fully characterize the specific protein binding domains of other protein interactions. Sulfo-SBED is the abbreviation for Sulfo-N-hydroxysuccinimidyl-2-(6-[biotinamido]-2-(p-azido benzamido)-hexanoamido) ethyl-1,3'-dithioproprionate. It is a heterobifunctional chemical crosslinker capable of covalently attaching to primary amines at one end and to nearly any protein functional group at the other end. Unlike typical crosslinkers, Sulfo-SBED also includes a biotin group and a cleavable disulfide spacer arm. Together these features allow one to sequentially crosslink interacting proteins and transfer the biotin affinity tag from one protein (i.e., a purified “bait” protein) to another (possibly unknown “prey” protein).

Highlights:

• Sulfo-NHS ester reacts with primary amines at pH 7-9
• Aryl azide conjugates randomly when activated with UV light
• Cleavable disulfide bond allows biotin label transfer
• Discover protein interactions without radiolabeling

Includes:

Sulfo-SBED No-Weigh™ Format 8 x 1mg microtubes, BupH™ Phosphate Buffered Saline 1 pack, Label Transfer Buffer (20X) 200mL, Streptavidin-Horseradish Peroxidase Conjugate 0.1mg, Dithiothreitol (DTT) No-Weigh Format 8 x 7.7mg microtubes, Slide-A-Lyzer™ MINI Dialysis Units Plus Float 10kDa MWCO 10 units/pack

Specifications

• Format: Kit
• Quantity: 8 Reactions
• Product Type: Crosslinker
• Solubility: Water
• Content And Storage: Sufficient For: 8 label transfer reactions for subsequent western blot analysis
  • Sulfo-SBED, 8 x 1 mg
  • PBS pack (makes 500 mL), 1 pack
  • Label Transfer Buffer (20X), 200 mL
  • Streptavidin-HRP Conjugate, 0.1 mg
  • Dithiothreitol (DTT), 8 x 7.7 mg
  • Slide-A-Lyzer MINI Dialysis Devices Plus Float, 10K MWCO, 10-device kit
  
  Store at 4°C
• Labeling Method: Chemical Labeling, Label Transfer
• Reactive Moiety: Sulfo-NHS Ester, Aryl Azide
• Product Line: Pierce™

For Research Use Only. Not for use in diagnostic procedures.