Thermo Scientific™ Pierce™ Ubiquitin Enrichment Kit

Description

Purify (enrich) poly-ubiquitinated proteins from cell and tissue lysates for post-translational modification studies using this affinity-resin, spin-column kit.

Thermo Scientific™ Pierce Ubiquitin Enrichment Kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.

The kit uses anti-ubiquitin affinity resin to bind and purify polyubiquitinated proteins (i.e., those with modifications containing more than four ubiquitin monomers) from cell lysates and other protein samples. The enriched fraction can subsequently be analyzed to determine the amount of general ubiquitin conjugates present or to identify a specific protein of interest by Western blotting. The assay protocol is fast and straight-forward, allowing for isolation of polyubiquitinated proteins and the fractionation of monoubiquitinated species in the resin flow-through. The Ubiquitin Enrichment Kit outperforms kits from other suppliers and provides a clean and specific preparation of proteins when compared to a control resin.

Highlights:

• Fast – less than 45 minutes hands-on time

• Complete – includes all reagents needed for ubiquitin-modified protein enrichment from cultured cells and tissue samples, as well as anti-ubiquitin antibody serum for Western blot analysis

• Flexible – sample incubation from two hours to overnight allows assay to be completed in several hours or in less than 30 minutes after overnight incubation

• Robust – compatible with all Thermo Scientific Pierce Cell Lysis Products and standard RIPA formulations

• Multiple-sample format – easily processes 1 to 15 samples concurrently

Includes:

Polyubiquitin affinity resin (300μL), Tris-buffered saline pack, microcentrifuge spin columns and accessories, polyubiquitin positive control and anti-ubiquitin rabbit anti-serum

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Specifications

• Content And Storage: Sufficient For: Enriching polyubiquitinated proteins from 15 lysate samples, each containing 0.15 mg total protein
  • Polyubiquitin Positive Control (1000X), 50 μL (store at -20°C)
  • Anti-ubiquitin Antibody (rabbit antiserum), 5 μL (store at -20°C)
  • Polyubiquitin Affinity Resin Slurry, 300 μL (store at 4°C—do not freeze)
  • TBS Pack (makes 500mL), 1 pack (store at 4°C)
  • Spin Columns and Accessories, 18 columns (store at 4°C)
• Product Line: Pierce™
• Quantity: 15 reactions kit
• Target: Polyubiquitinated Protein
• Type: Ubiquitin Enrichment Kit
• Stationary Phase: Proprietary Ligand
• Sufficient For: 15 Reactions
• Format: Kit, Microcentrifuge, Spin Column
• Column Type: Affinity, Agarose Resin

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.