Learn More
Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis
Description
Thermo Scientific Pierce Universal Nuclease for Cell Lysis is an endonuclease that is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.
Pierce Universal Nuclease for Cell Lysis is a genetically engineered endonuclease from Serratia marcescens. The enzyme is produced and purified from E. coli and consists of two identical 30-kDa subunits with two critical disulfide bonds. This indiscriminate endonuclease degrades single-stranded, double-stranded, linear and circular DNA and RNA and is effective over a wide range of temperatures and pH. This enzyme has very high specific activity (100-fold greater than DNase I) and has increased thermal stability compared to other nucleases. Pierce Universal Nuclease is ≥99% pure enzyme, is free of any measurable protease activity and is supplied at a concentration of 250 U/μL. Pierce Universal Nuclease for Cell Lysis is identical in performance to Benzonase™ Nuclease by EMD Merck™.
Highlights:
- Broad spectrum – degrades all forms of DNA and RNA
- Highest-quality enzyme – nuclease is at least 99% pure, as tested by SDS-PAGE
- Robust activity – 100-fold greater specific activity than DNase I
- Versatile – can be used with a wide variety of cell lysis reagents
Recommended for:
Use with B-PER, Y-PER or other commercial or homebrew cell lysis reagents and/or mechanical disruption to reduce viscosity in protein extracts; Remove DNA and RNA from recombinant protein preparations prior to downstream processing
Storage Requirements
Store in a cool, dry, well-ventilated area, protected from direct sunlight
Specifications
Specifications
| Product Type | Cell Lysis Enzyme |
| Content And Storage | Store in a cool, dry, well-ventilated area, protected from direct sunlight. |
| Form | Liquid |
| Product Line | Pierce |
| Buffer | Lysis Buffer |
| Reagent Type | Enzyme for Cell Lysis |
| Enzyme | Nuclease |
| Quantity | 25 kU |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.