Thermo Scientific™ Pierce™ Unstained Protein MW Marker

Description

A mixture of seven native proteins (14.4kDa to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Thermo Scientific™ Unstained Protein Molecular Weight Marker is a mixture of seven native proteins (14.4kDa to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

The protein MW marker is designed for accurate sizing of proteins in SDS-polyacrylamide gel electrophoresis, as well as on PVDF, nylon and nitrocellulose membranes. The protein standard produces sharp bands on protein mini gels following staining with Thermo Scientific Pierce Gel Stains or other coomassie dye or silver stains.

Highlights:

• Size range – seven proteins spanning 14.4 to 116kDa

• Ready-to-use – supplied in a loading buffer for direct loading on gels

• Sharp bands – equal intensity upon coomassie or silver staining

• Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins (0.1 to 0.2mg/mL of each) in 62.5mM Tris-HCl (pH 7.0 at 25°C), 1mM EDTA, 2% SDS, 50mM DTT, 30mM NaCl, 1mM NaN₃, 0.01% bromophenol blue and 50% glycerol; Component proteins: beta-galactosidase (116kDa), bovine serum albumin (66.2kDa), ovalbumin (45.0kDa), lactate dehydrogenase (35.0kDa), REase Bsp98I (25.0kDa), beta-lactoglobulin (18.4kDa), lysozyme (14.4kDa)

Recommended for:

Accurate protein sizing on SDS-polyacrylamide gels and Western blots

Specifications

• Content And Storage: Contents: two vials of 1 mL each
  
  Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 50 mM DTT, 30 mM NaCl, 0.01% bromophenol blue and 50% glycerol
  
  Storage: Upon receipt store at -20°C
  
  
• Number of Markers: 7
• Product Type: Protein Ladder
• Ready to Load: Yes
• Size Range: 14.4 to 116 kDa
• Stain Type: Unstained
• Gel Compatibility: Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
• Molecular Weight (g/mol): 116, 66.2, 45, 35, 25, 18.4, 14.4 kDa
• Product Line: Pierce™
• Quantity: 2 x 1 mL
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• System Type: Western Blotting, SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.